Strategy of tuning gene expression ratio in prokaryotic cell from perspective of noise and correlation

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	Strategy of tuning gene expression ratio in prokaryotic cell from perspective of noise and correlation
	Li, R; Xu, LF; Shi, HL
刊名	JOURNAL OF THEORETICAL BIOLOGY
	2015
卷号	365 期号:0 页码:377-389
关键词	Polar effect The two-state promoter Transcription terminator Gene expression correlation Operon structure
通讯作者	Shi, HL (reprint author), Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China.
英文摘要	Genes are organized into operons in procaryote, and these genes in one operon generally have related functions. However, genes in the same operon are usually not equally expressed, and the ratio needs to be fine-tuned for specific functions. We examine the difference of gene expression noise and correlation when tuning the expression level at the transcriptional or translational level in a bicistronic operon driven by a constitutive or a two-state promoter. We get analytic results for the noise and correlation of gene expression levels, which is confirmed by our stochastic simulations. Both the noise and the correlation of gene expressions in an operon with a two-state promoter are higher than in an operon with a constitutive promoter. Premature termination of mRNA induced by transcription terminator in the intergenic region or changing translation rates can tune the protein ratio at the transcriptional level or at the translational level. We find that gene expression correlation between promoter-proximal and promoter-distal genes at the protein level decreases as termination increases. In contrast, changing translation rates in the normal range almost does not alter the correlation. This explains why the translation rate is a key factor of modulating gene expressions in an operon. Our results can be useful to understand the relationship between the operon structure and the biological function of a gene network, and also may help in synthetic biology design. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Life Sciences & Biomedicine - Other Topics ; Mathematical & Computational Biology
类目[WOS]	Biology ; Mathematical & Computational Biology
关键词[WOS]	SHINE-DALGARNO SEQUENCE ; MESSENGER-RNA DECAY ; ESCHERICHIA-COLI ; BACILLUS-SUBTILIS ; SINGLE-CELL ; TRANSLATION ; CODON ; BACTERIA ; OPERON ; STOCHASTICITY
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.itp.ac.cn/handle/311006/21111]
专题	理论物理研究所_理论物理所1978-2010年知识产出
推荐引用方式 GB/T 7714	Li, R,Xu, LF,Shi, HL. Strategy of tuning gene expression ratio in prokaryotic cell from perspective of noise and correlation[J]. JOURNAL OF THEORETICAL BIOLOGY,2015,365(0):377-389.
APA	Li, R,Xu, LF,&Shi, HL.(2015).Strategy of tuning gene expression ratio in prokaryotic cell from perspective of noise and correlation.JOURNAL OF THEORETICAL BIOLOGY,365(0),377-389.
MLA	Li, R,et al."Strategy of tuning gene expression ratio in prokaryotic cell from perspective of noise and correlation".JOURNAL OF THEORETICAL BIOLOGY 365.0(2015):377-389.