In this study, we synthesized hydrophilic and hydrophobic polyacrylate resin using free-radical polymerization and embedded cupric ion source (Cu₂O) into the as-prepared resins to fabricate antifouling coatings, such of that the coatings combine the concept of “antifouling” and “fouling release” properties. In particular, the effect of hydrophobicity/hydrophilicity on anti-biofouling was investigated. The physicochemical properties of the coatings were characterized by infrared spectroscopy, atomic absorption spectroscopy, and contact angle measurements. Biofouling assays for the settlement and release of the diatom Navicula sp. and green alga Dunaliella tertiolecta were carried out under quasi-static and hydrodynamic condition. To prove the chemical stability and broad-spectrum antifouling characteristics of these coatings, the field tests were implemented in the South China Sea. Both results show that hydrophilic coating exhibits superior foul-resistance behavior to the hydrophobic coatings probably because of larger cupric ion release rate and the inhibition of hydrophobic interaction on hydrophilic coating.