Purification and characterization of a novel 1,3-propanediol oxidoreductase from Klebsiella oxytoca

	Purification and characterization of a novel 1,3-propanediol oxidoreductase from Klebsiella oxytoca
	Zhang, Gang 2,3; Yang, Guang 1; Li, Ying 2,3; Li, Jilun 2,3
刊名	AFRICAN JOURNAL OF BIOTECHNOLOGY
	2011-10-26
卷号	10 期号:66 页码:14913-14919
关键词	Klebsiella oxytoca 1,3-propanediol oxidoreductase purification YqhD
英文摘要	A novel 1,3-propanediol oxidoreductase (YqhD-1) found in Klebsiella oxytoca M5al was purified to homogeneity with a his-tag on a Ni-NTA column. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of the purified protein showed a molecular weight of 42 kDa. When YqhD-1 was tested as a dehydrogenase, the optimal pH was 11 and the optimal temperature was 37 C. Both Zn(2+) and Fe(2+) promoted YqhD-1 activity, whereas Mn(2+), Ni(2+), SO(4)(2-) and EDTA markedly inhibited YqhD-1 activity. Broad substrate specificity was observed for YqhD-1 with activity for several alcohols, including 1,3-propanediol, 1,2-propanediol, propanol, glycerol, 1-butanol, 1,3-butanediol and isopentanol. However, the enzyme preferred high carbon number alcohols such as 1-butanol and isopentanol. When tested as an aldehyde reductase, YqhD-1 could convert acetaldehyde and propaldehyde to their respective alcohols. When 1,3-PD and propaldehyde served as substrates, the apparent Km values of the enzyme for NADP and NADPH were 0.14 and 0.64 mM, respectively.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	CLOSTRIDIUM-BUTYRICUM E5 ; ESCHERICHIA-COLI ; ALCOHOL-DEHYDROGENASE ; GLYCEROL ; YQHD ; PNEUMONIAE ; ISOENZYME ; GENE
收录类别	SCI
语种	英语
WOS记录号	WOS:000298549800029
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1472]
专题	天津工业生物技术研究所_酶工程实验室马延和 _期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China 3.China Agr Univ, Coll Biol Sci, Dept Microbiol & Immunol, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Gang,Yang, Guang,Li, Ying,et al. Purification and characterization of a novel 1,3-propanediol oxidoreductase from Klebsiella oxytoca[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(66):14913-14919.
APA	Zhang, Gang,Yang, Guang,Li, Ying,&Li, Jilun.(2011).Purification and characterization of a novel 1,3-propanediol oxidoreductase from Klebsiella oxytoca.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(66),14913-14919.
MLA	Zhang, Gang,et al."Purification and characterization of a novel 1,3-propanediol oxidoreductase from Klebsiella oxytoca".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.66(2011):14913-14919.