Comparative genomic and proteomic analyses of Clostridium acetobutylicum Rh8 and its parent strain DSM 1731 revealed new understandings on butanol tolerance

	Comparative genomic and proteomic analyses of Clostridium acetobutylicum Rh8 and its parent strain DSM 1731 revealed new understandings on butanol tolerance
	Bao, Guanhui 1,2; Dong, Hongjun 1; Zhu, Yan 1; Mao, Shaoming 1; Zhang, Tianrui 1,4; Zhang, Yanping 1; Chen, Zugen 3; Li, Yin 1
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2014-08-08
卷号	450 期号:4 页码:1612-1618
关键词	Clostridium acetobutylicum Butanol tolerance Comparative genomics Comparative proteomics
英文摘要	Clostridium acetobutylicum strain Rh8 is a butanol-tolerant mutant which can tolerate up to 19 g/L butanol, 46% higher than that of its parent strain DSM 1731. We previously performed comparative cytoplasm- and membrane-proteomic analyses to understand the mechanism underlying the improved butanol tolerance of strain Rh8. In this work, we further extended this comparison to the genomic level. Compared with the genome of the parent strain DSM 1731, two insertion sites, four deletion sites, and 67 single nucleotide variations (SNVs) are distributed throughout the genome of strain Rh8. Among the 67 SNVs, 16 SNVs are located in the predicted promoters and intergenic regions; while 29 SNVs are located in the coding sequence, affecting a total of 21 proteins involved in transport, cell structure, DNA replication, and protein translation. The remaining 22 SNVs are located in the ribosomal genes, affecting a total of 12 rRNA genes in different operons. Analysis of previous comparative proteomic data indicated that none of the differentially expressed proteins have mutations in its corresponding genes. Rchange Algorithms analysis indicated that the mutations occurred in the ribosomal genes might change the ribosome RNA thermodynamic characteristics, thus affect the translation strength of these proteins. Take together, the improved butanol tolerance of C acetobutylicum strain Rh8 might be acquired through regulating the translational process to achieve different expression strength of genes involved in butanol tolerance. (C) 2014 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	RIBOSOMAL-RNA ; SEQUENCE ; ALGORITHMS ; BUTYRATE ; STRESS ; MAP
收录类别	SCI
语种	英语
WOS记录号	WOS:000341338100062
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1344]
专题	天津工业生物技术研究所_酶工程实验室马延和 _期刊论文
作者单位	1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Calif Los Angeles, Sch Med, Dept Human Genet, Los Angeles, CA 90095 USA 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China
推荐引用方式 GB/T 7714	Bao, Guanhui,Dong, Hongjun,Zhu, Yan,et al. Comparative genomic and proteomic analyses of Clostridium acetobutylicum Rh8 and its parent strain DSM 1731 revealed new understandings on butanol tolerance[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,450(4):1612-1618.
APA	Bao, Guanhui.,Dong, Hongjun.,Zhu, Yan.,Mao, Shaoming.,Zhang, Tianrui.,...&Li, Yin.(2014).Comparative genomic and proteomic analyses of Clostridium acetobutylicum Rh8 and its parent strain DSM 1731 revealed new understandings on butanol tolerance.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,450(4),1612-1618.
MLA	Bao, Guanhui,et al."Comparative genomic and proteomic analyses of Clostridium acetobutylicum Rh8 and its parent strain DSM 1731 revealed new understandings on butanol tolerance".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 450.4(2014):1612-1618.