Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-beta-1,4-xylanase

CORC > 天津工业生物技术研究所 > 结构生物学与蛋白酶学实验室郭瑞庭 > 期刊论文

	Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-beta-1,4-xylanase
	Zheng, Yingying 1; Li, Yujie 1; Liu, Weidong 1; Chen, Chun-Chi 1; Ko, Tzu-Ping 2; He, Miao 1,4; Xu, Zhongxia 1,5; Liu, Meixia 1,4; Luo, Huiying 3; Guo, Rey-Ting 1
刊名	JOURNAL OF STRUCTURAL BIOLOGY
	2016-03-01
卷号	193 期号:3 页码:206-211
关键词	Xylanase Xylobiose Psychrophilic Cold-adaptation
英文摘要	The cold-adapted xylanases can catalyze at low temperature and hold great potential in food industry applications. Here we describe the first crystal structure of a cold-adapted glycoside hydrolase (GH) family 10 xylanase XynGR40 and its complex with xylobiose at 2.15 and 2.50 angstrom resolution. The enzyme folds into a typical GH10 (beta/alpha)(8) TIM-barrel, with E132 and E243 serving as the catalytic residues. The xylobiose was observed to occupy the -1 and -2 subsites. Structural comparison with a thermophilic GH10 xylanase highlighting various parameters that may explain the cold adaptation features were analyzed. Synergistic effects of the increased exposure of hydrophobic residues, the higher flexibility of substrate-binding residues, more flexible loops, and the ratios of special amino acid residues, may result in the cold adaptation of XynGR40. (C) 2015 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]	ANGSTROM RESOLUTION ; INDUSTRIAL APPLICATIONS ; CRYSTAL-STRUCTURE ; ALPHA-AMYLASE ; ACTIVE-SITE ; 11 XYLANASE ; THERMOSTABILITY ; INTERMEDIATE ; SOFTWARE ; SEQUENCE
收录类别	SCI
语种	英语
WOS记录号	WOS:000370911500007
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1539]
专题	天津工业生物技术研究所_结构生物学与蛋白酶学实验室郭瑞庭_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 2.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 3.Chinese Acad Agr Sci, Feed Res Inst, Key Lab Feed Biotechnol, Minist Agr, Beijing 100081, Peoples R China 4.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China 5.Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Yingying,Li, Yujie,Liu, Weidong,et al. Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-beta-1,4-xylanase[J]. JOURNAL OF STRUCTURAL BIOLOGY,2016,193(3):206-211.
APA	Zheng, Yingying.,Li, Yujie.,Liu, Weidong.,Chen, Chun-Chi.,Ko, Tzu-Ping.,...&Ma, Yanhe.(2016).Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-beta-1,4-xylanase.JOURNAL OF STRUCTURAL BIOLOGY,193(3),206-211.
MLA	Zheng, Yingying,et al."Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-beta-1,4-xylanase".JOURNAL OF STRUCTURAL BIOLOGY 193.3(2016):206-211.