Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor

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	Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor
	Zheng, Yingying 1; Ko, Tzu-Ping 2; Yang, Yunyun 1,3; Shao, Weilan 4; Guo, Rey-Ting 1
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2015-01-16
卷号	456 期号:3 页码:733-736
关键词	Transcription regulation Ethanol fermentation Thennoanaerobacter ethanolicus Allosteric effector
英文摘要	The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). RSP is the homologue of Rex in Thermoanaerobacter ethanolicus JW200(T) and regulates ethanol fermentation in this obligate anaerobe. The dimeric repressor binds to DNA by an open conformation. The crystal structure of RSP/alpha-NAD(+) complex shows a different set of ligand interactions mainly due to the unique configuration of the nicotinamide moiety. The positively charged ring is covered by the Tyr102 side chain and interacts with a sulfate ion adjacent to the N-terminus of helix alpha 8. Consequently, the RSP dimer may be locked in a closed conformation that does not bind to DNA. However, alpha-NAD(+) does not show a higher affinity to RSP than B-NAD+. It has to be improved for possible use as an effector in modulating the repressor. (C) 2014 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	REDOX ; CRYSTALLOGRAPHY ; FEATURES
收录类别	SCI
语种	英语
WOS记录号	WOS:000348973300005
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1429]
专题	天津工业生物技术研究所_结构生物学与蛋白酶学实验室郭瑞庭_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 2.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 3.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China 4.Jiangsu Univ, Biofuels Inst, Sch Environm, Zhenjiang 212013, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Yingying,Ko, Tzu-Ping,Yang, Yunyun,et al. Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,456(3):733-736.
APA	Zheng, Yingying,Ko, Tzu-Ping,Yang, Yunyun,Shao, Weilan,&Guo, Rey-Ting.(2015).Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,456(3),733-736.
MLA	Zheng, Yingying,et al."Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 456.3(2015):733-736.