One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes

	One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes
	Zhou, Wei 1; You, Chun 2; Ma, Hongwu 1; Ma, Yanhe 1; Zhang, Y. -H. Percival 1,2,3
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2016-03-02
卷号	64 期号:8 页码:1777-1783
关键词	Camellia sinensis hydroperoxide lyase Ectropis obliqua suppressive subtractive hybridization (SSH) induced defense response
英文摘要	Hydroperoxide lyase (HPL, E.C. 4.1.2.) is the major enzyme in the biosynthesis of natural volatile aldehydes and alcohols in plants, however, little was known about HPL in tea plants (Camellia sinensis). A unique cDNA fragment was isolated by suppressive subtractive hybridization (SSH) from a tea plant subjected to herbivory by tea geometrid Ectropis obliqua. This full length cDNA acquired by RACE was 1476 bp and encoded 491 amino acids. DNA and protein BLAST searches showed high homology to HPL sequences from other plants. The His-tag expression vector pET-32a(+)/CsHPL was constructed and transferred into Escherichia coli Rosetta (DE3). The expression product of recombinant CsHPL in E. coli was about 60 kDa. The enzyme activity of CsHPL was 0.20 mu mol.min(-1).mg(-1). Quantitative RT-PCR analysis indicated CsHPL was strongly up-regulated in tea plants after Ectropis obliqua attack, suggesting that it may be an important candidate for defense against insects in tea plants.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]	Agriculture ; Chemistry ; Food Science & Technology
关键词[WOS]	ARCHAEON THERMOCOCCUS-LITORALIS ; ESCHERICHIA-COLI ; MALTODEXTRIN-PHOSPHORYLASE ; GLUCAN PHOSPHORYLASE ; THERMOSTABLE 4-ALPHA-GLUCANOTRANSFERASE ; LEUCONOSTOC-MESENTEROIDES ; SUCROSE PHOSPHORYLASE ; THERMUS-THERMOPHILUS ; PYROCOCCUS-FURIOSUS ; SULFOLOBUS-TOKODAII
收录类别	SCI
语种	英语
WOS记录号	WOS:000371454000015
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1527]
专题	天津工业生物技术研究所_计算生物设计马洪武 _期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, 32 West Seventh Ave, Tianjin 300308, Peoples R China 2.Cell Free Bioinnovat Inc, 1800 Kraft Dr,Suite 222, Blacksburg, VA 24060 USA 3.Virginia Tech, Dept Biol Syst Engn, 304 Seitz Hall, Blacksburg, VA 24061 USA
推荐引用方式 GB/T 7714	Zhou, Wei,You, Chun,Ma, Hongwu,et al. One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016,64(8):1777-1783.
APA	Zhou, Wei,You, Chun,Ma, Hongwu,Ma, Yanhe,&Zhang, Y. -H. Percival.(2016).One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,64(8),1777-1783.
MLA	Zhou, Wei,et al."One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.8(2016):1777-1783.