Comparative genome analysis of a thermotolerant Escherichia coli obtained by Genome Replication Engineering Assisted Continuous Evolution (GREACE) and its parent strain provides new understanding of microbial heat tolerance

	Comparative genome analysis of a thermotolerant Escherichia coli obtained by Genome Replication Engineering Assisted Continuous Evolution (GREACE) and its parent strain provides new understanding of microbial heat tolerance
	Luan, Guodong 1,2; Bao, Guanhui 1,2; Lin, Zhao 1,2; Li, Yang 3; Chen, Zugen 3,4; Li, Yin 1; Cai, Zhen 1
刊名	NEW BIOTECHNOLOGY
	2015-12-25
卷号	32 期号:6 页码:732-738
英文摘要	Heat tolerance of microbes is of great importance for efficient biorefinery and bioconversion. However, engineering and understanding of microbial heat tolerance are difficult and insufficient because it is a complex physiological trait which probably correlates with all gene functions, genetic regulations, and cellular metabolisms and activities. In this work, a novel strain engineering approach named Genome Replication Engineering Assisted Continuous Evolution (GREACE) was employed to improve the heat tolerance of Escherichia coli. When the E. coli strain carrying a mutator was cultivated under gradually increasing temperature, genome-wide mutations were continuously generated during genome replication and the mutated strains with improved thermotolerance were autonomously selected. A thermotolerant strain HR50 capable of growing at 50 degrees C on LB agar plate was obtained within two months, demonstrating the efficiency of GREACE in improving such a complex physiological trait. To understand the improved heat tolerance, genomes of HR50 and its wildtype strain DH5 alpha were sequenced. Evenly distributed 361 mutations covering all mutation types were found in HR50. Closed material transportations, loose genome conformation, and possibly altered cell wall structure and transcription pattern were the main differences of HR50 compared with DH5 alpha, which were speculated to be responsible for the improved heat tolerance. This work not only expanding our understanding of microbial heat tolerance, but also emphasizing that the in vivo continuous genome mutagenesis method, GREACE, is efficient in improving microbial complex physiological trait.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	BUTANOL TOLERANCE ; GENE-EXPRESSION ; ACID TOLERANCE ; DSM 1731 ; DNA ; RESISTANCE ; BACTERIA ; IMPROVEMENT ; POLYMERASE ; POLYAMINES
收录类别	SCI
语种	英语
WOS记录号	WOS:000360920400029
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1523]
专题	天津工业生物技术研究所_基因组分析实验室陈祖耕_期刊论文
作者单位	1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 4.Univ Calif Los Angeles, Sch Med, Dept Human Genet, Los Angeles, CA 90095 USA
推荐引用方式 GB/T 7714	Luan, Guodong,Bao, Guanhui,Lin, Zhao,et al. Comparative genome analysis of a thermotolerant Escherichia coli obtained by Genome Replication Engineering Assisted Continuous Evolution (GREACE) and its parent strain provides new understanding of microbial heat tolerance[J]. NEW BIOTECHNOLOGY,2015,32(6):732-738.
APA	Luan, Guodong.,Bao, Guanhui.,Lin, Zhao.,Li, Yang.,Chen, Zugen.,...&Cai, Zhen.(2015).Comparative genome analysis of a thermotolerant Escherichia coli obtained by Genome Replication Engineering Assisted Continuous Evolution (GREACE) and its parent strain provides new understanding of microbial heat tolerance.NEW BIOTECHNOLOGY,32(6),732-738.
MLA	Luan, Guodong,et al."Comparative genome analysis of a thermotolerant Escherichia coli obtained by Genome Replication Engineering Assisted Continuous Evolution (GREACE) and its parent strain provides new understanding of microbial heat tolerance".NEW BIOTECHNOLOGY 32.6(2015):732-738.