Optimized expression of (S)-carbonyl reductase in Pichia pastoris for efficient production of (S)-1-phenyl-1, 2-ethanediol

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	Optimized expression of (S)-carbonyl reductase in Pichia pastoris for efficient production of (S)-1-phenyl-1, 2-ethanediol
	Zhang, Rongzhen 1,2,3; Xu, Yan 1,2,3; Xiao, Rong 4; Wang, Lei 1,2; Zhang, Botao 1,2,5
刊名	JOURNAL OF BASIC MICROBIOLOGY
	2014-08-01
卷号	54 期号:8 页码:873-879
关键词	Candida parapsilosis (S)-carbonyl reductase Pichia pastoris Expression Chiral alcohol
英文摘要	The recombinant (S)-carbonyl reductase (SCR) in Escherichia coli catalyzed the reduction of 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol (PED) with low efficiency. In this work, its 6x histidine fusion gene his(6)-scr was cloned in Pichia pastoris under the control of the AOX1 methanol inducible promoter. The heterologous protein SCR was expressed through a Mut(s) phenotype. Under the optimal conditions: pH 7.0, initial OD600 2.5, methanol daily addition concentration 1.0% and induction duration 4-5 days, the recombinant protein SCR was produced at the highest level. The enzyme activity in the cell-free exacts of P. pastoris was 0.38, which was over twofold than that of the recombinant E. coli-SCR. The enzyme was purified to homogeneity with a specific activity of 3.41 U mg(-1), and it catalyzed the biotransformation of (S)-PED with a high optical purity of 96.9% in a high yield of 89.7% at optimum pH of 7.0. The developed effective system of P. pastoris-SCR will facilitate the preparation of pure chiral alcohol in industry.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	ANTI-PRELOG STEREOSPECIFICITY ; CANDIDA-PARAPSILOSIS ; CARBONYL REDUCTASE ; PROTEIN EXPRESSION ; ESCHERICHIA-COLI ; INDUSTRIAL BIOCATALYSIS ; GENE ; DEHYDROGENASE ; YEAST ; (R)-1-PHENYL-1,2-ETHANEDIOL
收录类别	SCI
语种	英语
WOS记录号	WOS:000342822700014
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1401]
专题	天津工业生物技术研究所_基因工程与微生物应用技术研究组宋恢_期刊论文
作者单位	1.Jiangnan Univ, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Peoples R China 2.Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China 3.Jiangnan Univ, Natl Key Lab Food Sci, Wuxi 214122, Peoples R China 4.Rutgers State Univ, Ctr Adv Biotechnol & Med, Piscataway, NJ 08854 USA 5.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Rongzhen,Xu, Yan,Xiao, Rong,et al. Optimized expression of (S)-carbonyl reductase in Pichia pastoris for efficient production of (S)-1-phenyl-1, 2-ethanediol[J]. JOURNAL OF BASIC MICROBIOLOGY,2014,54(8):873-879.
APA	Zhang, Rongzhen,Xu, Yan,Xiao, Rong,Wang, Lei,&Zhang, Botao.(2014).Optimized expression of (S)-carbonyl reductase in Pichia pastoris for efficient production of (S)-1-phenyl-1, 2-ethanediol.JOURNAL OF BASIC MICROBIOLOGY,54(8),873-879.
MLA	Zhang, Rongzhen,et al."Optimized expression of (S)-carbonyl reductase in Pichia pastoris for efficient production of (S)-1-phenyl-1, 2-ethanediol".JOURNAL OF BASIC MICROBIOLOGY 54.8(2014):873-879.