Mutagenesis and evaluation of cellulase properties and cellulose hydrolysis of Talaromyces piceus

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	Mutagenesis and evaluation of cellulase properties and cellulose hydrolysis of Talaromyces piceus
	He, Ronglin 1,2,3; Cai, Pengli 1; Wu, Gaihong 1; Zhang, Can 1; Zhang, Dongyuan 1; Chen, Shulin 1
刊名	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
	2015-11-01
卷号	31 期号:11 页码:1811-1819
关键词	beta-glucosidase Strain improvement Cellulase Hydrolysis
英文摘要	A fungal species with a high yield of beta-glucosidase was isolated and identified as Talaromyces piceus 9-3 (anamorph: Penicillium piceum) by morphological and molecular characterization. Through dimethyl sulphate mutagenesis, the cellulase over-producing strain T. piceus H16 was obtained. The FPase activity and beta-glucosidase activity of T. piceus H16 were 5.83 and 53.12 IU ml(-1) respectively-a 5.34- and 4.43-times improvement from the parent strain T. piceus 9-3. The optimum pH and temperature for enzyme activity were pH 5.0 and 50 A degrees C for FPase activity and pH 5.0 and 55 A degrees C for beta-glucosidase activity, respectively. The cellulase were quite stable at 37 A degrees C, only losing < 10 % of their initial activity after 24 h of incubation. Hydrolysis analysis results showed that a highly efficient synergistic effect was achieved by combining cellulase from T. piceus H16 with that from Trichoderma reesei RUT C30 on hydrolyzing different substrates due to the high beta-glucosidase activity of T. piceus H16. These data suggest that T. piceus H16 can be used as a potential cellulase producer with good prospects.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	PENICILLIUM-ECHINULATUM ; TRICHODERMA-REESEI ; BETA-GLUCOSIDASE ; STRAIN ; IMPROVEMENT ; CHALLENGES ; STRATEGIES ; INDUCTION ; XYLANASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000361822300017
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1576]
专题	天津工业生物技术研究所_工业生物系统工程实验室陈树林_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Tianjin Univ Sci & Technol, Minist Educ, Key Lab Ind Fermentat Microbiol, Tianjin 300457, Peoples R China 3.Tianjin Univ Sci & Technol, Tianjin Key Lab Ind Microbiol, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	He, Ronglin,Cai, Pengli,Wu, Gaihong,et al. Mutagenesis and evaluation of cellulase properties and cellulose hydrolysis of Talaromyces piceus[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2015,31(11):1811-1819.
APA	He, Ronglin,Cai, Pengli,Wu, Gaihong,Zhang, Can,Zhang, Dongyuan,&Chen, Shulin.(2015).Mutagenesis and evaluation of cellulase properties and cellulose hydrolysis of Talaromyces piceus.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,31(11),1811-1819.
MLA	He, Ronglin,et al."Mutagenesis and evaluation of cellulase properties and cellulose hydrolysis of Talaromyces piceus".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 31.11(2015):1811-1819.