Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production

CORC > 天津工业生物技术研究所 > 功能糖与天然活性物质研究组孙媛霞 > 期刊论文

	Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production
	Zhu, Yueming 1; Li, Hongyi 2; Liu, Pingping 1; Yang, Jiangang 1; Zhang, Xueli 1; Sun, Yuanxia 1
刊名	JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
	2015-05-01
卷号	42 期号:5 页码:661-669
关键词	Allitol Multi-enzyme coupling pathway Cofactor recycling system Whole-cell biotransformation
英文摘要	An engineered strain for the conversion of d-fructose to allitol was developed by constructing a multi-enzyme coupling pathway and cofactor recycling system in Escherichia coli. d-Psicose-3-epimerase from Ruminococcus sp. and ribitol dehydrogenase from Klebsiella oxytoca were coexpressed to form the multi-enzyme coupling pathway for allitol production. The cofactor recycling system was constructed using the formate dehydrogenase gene from Candida methylica for continuous NADH supply. The recombinant strain produced 10.62 g/l allitol from 100 mM d-fructose. To increase the intracellular concentration of the substrate, the glucose/fructose facilitator gene from Zymomonas mobilis was incorporated into the engineered strain. The results showed that the allitol yield was enhanced significantly to 16.53 g/l with a conversion rate of 92 %. Through optimizing conversion conditions, allitol was produced effectively on a large scale by the whole-cell biotransformation system; the yield reached 48.62 g/l when 500 mM d-fructose was used as the substrate.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	D-TAGATOSE 3-EPIMERASE ; WHOLE-CELL BIOTRANSFORMATION ; GLUCOSE-DEHYDROGENASE GENES ; AGGLOMERANS STRAIN 221E ; D-PSICOSE 3-EPIMERASE ; D-MANNITOL FORMATION ; D-FRUCTOSE ; ETHYL (S)-4-CHLORO-3-HYDROXYBUTANOATE ; RIBITOL DEHYDROGENASE ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000352860600001
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1459]
专题	天津工业生物技术研究所_功能糖与天然活性物质研究组孙媛霞 _期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China 2.Shanxi Med Univ, Key Lab Cellular Physiol, Minist Educ, Taiyuan 030001, Shanxi, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Yueming,Li, Hongyi,Liu, Pingping,et al. Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2015,42(5):661-669.
APA	Zhu, Yueming,Li, Hongyi,Liu, Pingping,Yang, Jiangang,Zhang, Xueli,&Sun, Yuanxia.(2015).Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,42(5),661-669.
MLA	Zhu, Yueming,et al."Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 42.5(2015):661-669.