Structural vaccinology: structure-based design of influenza A virus hemagglutinin subtype-specific subunit vaccines

CORC > 天津工业生物技术研究所 > 蛋白质工程与疫苗实验室高福 > 期刊论文

	Structural vaccinology: structure-based design of influenza A virus hemagglutinin subtype-specific subunit vaccines
	Xuan, Chunling 1,2; Shi, Yi 1; Qi, Jianxun 1; Zhang, Wei 1,2; Xiao, Haixia 4; Gao, George F.1,2,3,4
刊名	PROTEIN & CELL
	2011-12-01
卷号	2 期号:12 页码:997-1005
关键词	influenza virus subunit vaccine hemagglutinin structure design
英文摘要	There is a great need for new vaccine development against influenza A viruses due to the drawbacks of traditional vaccines that are mainly prepared using embryonated eggs. The main component of the current split influenza A virus vaccine is viral hemagglutinin (HA) which induces a strong antibody-mediated immune response. To develop a modern vaccine against influenza A viruses, the current research has been focused on the universal vaccines targeting viral M2, NP and HA proteins. Crystallographic studies have shown that HA forms a trimer embedded on the viral envelope surface, and each monomer consists of a globular head (HA1) and a "rod-like" stalk region (HA2), the latter being more conserved among different HA subtypes and being the primary target for universal vaccines. In this study, we rationally designed the HA head based on the crystal structure of the 2009-pandemic influenza A (H1N1) virus HA as a model, tested its immunogenicity in mice, solved its crystal structure and further examined its immunological characteristics. The results show that the HA globular head can be easily prepared by in vitro refolding in an E. coli expression system, which maintains its intact structure and allows for the stimulation of a strong immune response. Together with recent reports on some similar HA globular head preparations we conclude that structure-based rational design of the HA globular head can be used for subtype-specific vaccines against influenza viruses.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Cell Biology
研究领域[WOS]	Cell Biology
关键词[WOS]	RECEPTOR-BINDING ; ANTIGENIC STRUCTURE ; PANDEMIC INFLUENZA ; MAXIMUM-LIKELIHOOD ; MEMBRANE-FUSION ; ORIGIN ; VACCINATION ; ANTIBODIES ; INFECTION ; EPITOPE
收录类别	SCI
语种	英语
WOS记录号	WOS:000310522900008
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/1473]
专题	天津工业生物技术研究所_蛋白质工程与疫苗实验室高福_期刊论文
作者单位	1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ Beijing, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Beijing Inst Life Sci, Res Network Immun & Hlth RNIH, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Lab Prot Engn & Vaccines, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Xuan, Chunling,Shi, Yi,Qi, Jianxun,et al. Structural vaccinology: structure-based design of influenza A virus hemagglutinin subtype-specific subunit vaccines[J]. PROTEIN & CELL,2011,2(12):997-1005.
APA	Xuan, Chunling,Shi, Yi,Qi, Jianxun,Zhang, Wei,Xiao, Haixia,&Gao, George F..(2011).Structural vaccinology: structure-based design of influenza A virus hemagglutinin subtype-specific subunit vaccines.PROTEIN & CELL,2(12),997-1005.
MLA	Xuan, Chunling,et al."Structural vaccinology: structure-based design of influenza A virus hemagglutinin subtype-specific subunit vaccines".PROTEIN & CELL 2.12(2011):997-1005.