A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 仿生智能材料团队

	A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
	Liu, Ge 1,2,3; Kuang, Shan 1,2; Wu, Shimei 4; Jin, Weihua 1,2; Sun, Chaomin 1,2
刊名	SCIENTIFIC REPORTS
	2016-05-24
卷号	6
英文摘要	Many polysaccharides isolated from plants have exhibited promising antitumor activities. The aim of this study is to investigate the antitumor activity of the novel polysaccharide named SPS from Sargassum integerrimum, elucidate the underlying anticancer mechanism in a human lung cancer cell line A549, and evaluate its anti-angiogenic activity both in vitro and in vivo. The results show that SPS significantly reduces A549 cells viability in a dose-and time-dependent manner via MTT method. Flow cytometry analysis indicates that SPS could induce cell apoptosis, the loss of mitochondrial membrane potential (MMP), generation of reactive oxygen species (ROS) and G2/M phase cell cycle arrest of A549 cells. Up-regulation of the expressions of P53 and Bax, down-regulation of the expression of Bcl-2, and activation of cleaved caspase-3, caspase-9 and PARP are also detected by western blotting after the treatment of SPS. In addition, SPS inhibits the proliferation, migration and cord formation of human umbilical vein endothelial cells (HUVECs) in vitro, and prevents the vascular development of zebrafish embryos in vivo. Altogether, our data prove the anticancer and anti-angiogenesis properties of SPS, and provide further insights into the potential pharmacological application of SPS as antitumor and antiangiogenic agent against lung cancer.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	ENDOTHELIAL-CELLS ; BCL-2 FAMILY ; LUNG-CANCER ; ANTIOXIDANT ACTIVITIES ; INHIBITS ANGIOGENESIS ; MECHANISMS ; ANTITUMOR ; ROS ; ANTIANGIOGENESIS ; PROLIFERATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000376390900001
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/8278]
专题	青岛生物能源与过程研究所_仿生智能材料团队
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ge,Kuang, Shan,Wu, Shimei,et al. A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo[J]. SCIENTIFIC REPORTS,2016,6.
APA	Liu, Ge,Kuang, Shan,Wu, Shimei,Jin, Weihua,&Sun, Chaomin.(2016).A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo.SCIENTIFIC REPORTS,6.
MLA	Liu, Ge,et al."A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo".SCIENTIFIC REPORTS 6(2016).