Cloning and characterization of Bax1 and Bax2 genes of Ctenopharyngodon idellus and evaluation of transcript expression in response to grass carp reovirus infection

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	Cloning and characterization of Bax1 and Bax2 genes of Ctenopharyngodon idellus and evaluation of transcript expression in response to grass carp reovirus infection
	Wang, Hao 1,2; He, Libo 1; Pei, Yongyan 1,2; Chu, Pengfei 1,2; Huang, Rong 1; Li, Yongming 1; Liao, Lanjie 1; Zhu, Zuoyan 1; Wang, Yaping 1
刊名	FISH PHYSIOLOGY AND BIOCHEMISTRY
	2016-10-01
卷号	42 期号:5 页码:1369-1382
关键词	Grass carp Bax GCRV Apoptosis qRT-PCR
ISSN号	0920-1742
英文摘要	Multidomain proapoptotic Bcl-2-associated X (Bax) protein is an essential effector responsible for mitochondrial outer membrane permeabilization, resulting in cell death via apoptosis. In this study, two Bax genes of grass carp (Ctenopharyngodon idellus), designated as CiBax1 and CiBax2, were isolated and analyzed. The obtained CiBax1 cDNA is 2058 bp long, with a 579 bp open reading frame (ORF) coding a protein of 192 amino acid residues. The full-length cDNA of CiBax2 is 1161 bp, with a 618 bp ORF coding 205 amino acids. Both CiBax1 and CiBax2 are typical members of Bcl-2 family containing conserved Bcl and C-terminal domains, and they share conserved synteny with zebrafish Bax genes despite the grass carp Bax mapping to different linkage groups. Phylogenetic analysis showed that CiBax1 was clustered with Bax from most teleost fish, and CiBax2 was close to Bax2 from teleost fish but far separated from that of Salmo salar. Quantitative real-time PCR analysis revealed broad expression of CiBax1 and CiBax2 in tissues from healthy grass carp, but the relative expression level differed. The mRNA expression of CiBax1 and CiBax2 was both upregulated significantly and peaked in all examined tissues at days 5 or 6 post-infection with grass carp reovirus. Subcellular localization indicated that CiBax1 protein was localized in both nucleus and cytosol, while CiBax2 protein only in cytosol. Moreover, CiBax2, but not CiBax1 was colocalized with mitochondrion under normal condition. Taken together, the findings would be helpful for further understanding of the function of Bax in teleost fish.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Fisheries ; Physiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Fisheries ; Physiology
关键词[WOS]	BCL-2 PROTEIN FAMILY ; INDUCED APOPTOSIS ; CELL-DEATH ; SUBCELLULAR-LOCALIZATION ; MITOCHONDRIAL APOPTOSIS ; ENDOPLASMIC-RETICULUM ; OUTER-MEMBRANE ; ZEBRAFISH ; BAK ; REPLICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000383355700010
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28363]
专题	水生生物研究所_淡水生态学研究中心_学位论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hao,He, Libo,Pei, Yongyan,et al. Cloning and characterization of Bax1 and Bax2 genes of Ctenopharyngodon idellus and evaluation of transcript expression in response to grass carp reovirus infection[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2016,42(5):1369-1382.
APA	Wang, Hao.,He, Libo.,Pei, Yongyan.,Chu, Pengfei.,Huang, Rong.,...&Wang, Yaping.(2016).Cloning and characterization of Bax1 and Bax2 genes of Ctenopharyngodon idellus and evaluation of transcript expression in response to grass carp reovirus infection.FISH PHYSIOLOGY AND BIOCHEMISTRY,42(5),1369-1382.
MLA	Wang, Hao,et al."Cloning and characterization of Bax1 and Bax2 genes of Ctenopharyngodon idellus and evaluation of transcript expression in response to grass carp reovirus infection".FISH PHYSIOLOGY AND BIOCHEMISTRY 42.5(2016):1369-1382.