NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-kappa B, and also MDA5, RIG-I and MAVS

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	NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-kappa B, and also MDA5, RIG-I and MAVS
	Zou, Peng Fei 1,2; Chang, Ming Xian 2; Li, Ying 3; Xue, Na Na 2; Li, Jun Hua 2; Chen, Shan Nan 2; Nie, Pin 1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2016-08-01
卷号	55 页码:173-185
关键词	Nod-like receptor NOD2 RLR NF-kappaB Zebrafish
ISSN号	1050-4648
英文摘要	NOD2/RIPK2 signalling plays essential role in the modulation of innate and adaptive immunity in mammals. In this study, NOD2 was functionally characterized in zebrafish (Danio rerio), and its interaction with a receptor-interaction protein, RIPK2, and RLRs such as MDA5 and RIG-I, as well as the adaptor, MAVS was revealed in fish innate immunity. The expression of NOD2 and RIPK2 in ZF4 cells has been constitutive and can be induced by the infection of Edwardsiella tarda and SVCV. The NOD2 can sense MDP in PGN from Gram-negative and-positive bacteria. It is further revealed that the NOD2 and RIPK2 can activate NF-kappa B and IFN promoters, inducing significantly antiviral defense against SVCV infection. As observed in the reduced bacterial burden in RIPK2 overexpressed cells, RIPK2 also has a role in inhibiting the bacterial replication. The overexpression of NOD2 in zebrafish embryos resulted in the increase of immune gene expression, especially those encoding PRRs and cytokines involved in antiviral response such as MDA5, RIG-I, and type I IFNs, etc. Luciferase reporter assays and co-immunoprecipitation assays demonstrated that zebrafish NOD2 is associated with MDA5 and RIG-I in signalling pathway. In addition, it is further demonstrated that RIPK2 and MAVS in combination with NOD2 have an enhanced role in "NOD2-mediated NF-kappa B and type I IFN activation. It is concluded that teleost fish NOD2 can not only sense MDP for activating innate immunity as reported in mammals, but can also interact with other PRRs to form a network in antiviral innate response. (C) 2016 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	INNATE IMMUNE-RESPONSES ; SIGNALING PATHWAYS ; PATHOGEN RECOGNITION ; HOST RECOGNITION ; CARD15 MUTATIONS ; BLAU-SYNDROME ; ACTIVATION ; GENE ; INDUCTION ; PEPTIDOGLYCAN
收录类别	SCI
语种	英语
WOS记录号	WOS:000381537000017
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28343]
专题	水生生物研究所_藻类生物学及应用研究中心_学位论文
作者单位	1.Jimei Univ, Coll Fisheries, 43 Yindou Rd, Xiamen 361021, Fujian Province, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 3.Xiamen Univ, Tan Kah Kee Coll, Dept Environm Sci & Engn, Zhangzhou 363105, Fujian Province, Peoples R China
推荐引用方式 GB/T 7714	Zou, Peng Fei,Chang, Ming Xian,Li, Ying,et al. NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-kappa B, and also MDA5, RIG-I and MAVS[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:173-185.
APA	Zou, Peng Fei.,Chang, Ming Xian.,Li, Ying.,Xue, Na Na.,Li, Jun Hua.,...&Nie, Pin.(2016).NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-kappa B, and also MDA5, RIG-I and MAVS.FISH & SHELLFISH IMMUNOLOGY,55,173-185.
MLA	Zou, Peng Fei,et al."NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-kappa B, and also MDA5, RIG-I and MAVS".FISH & SHELLFISH IMMUNOLOGY 55(2016):173-185.