Comparative genomics analyses on EPS biosynthesis genes required for floc formation of Zoogloea resiniphila and other activated sludge bacteria

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	Comparative genomics analyses on EPS biosynthesis genes required for floc formation of Zoogloea resiniphila and other activated sludge bacteria
	An, Weixing 1,2; Guo, Feng 3; Song, Yulong 1,2; Gao, Na 1,2; Bai, Shijie 1,7; Dai, Jingcheng 1; Wei, Hehong 1,2; Zhang, Liping 1; Yu, Dianzhen 1,2; Xia, Ming 1,2
刊名	WATER RESEARCH
	2016-10-01
卷号	102 页码:494-504
关键词	Genome Zoogloea Activated sludge Floc formation Asparagine synthases
ISSN号	0043-1354
英文摘要	Activated sludge (AS) process has been widely utilized for municipal sewage and industrial wastewater treatment. Zoolgoea and its related floc-forming bacteria are required for formation of AS flocs which is the key to gravitational effluent-and-sludge separation and AS recycling. However, little is known about the genetics, biochemistry and physiology of Zoogloea and its related bacteria. This report deals with the comparative genomic analyses on two Zoogloea resiniphila draft genomes and the closely related proteobacterial species commonly found in AS. In particular, the metabolic processes involved in removal of organic matters, nitrogen and phosphorus were analyzed. Furthermore, it is revealed that a large gene cluster, encoding eight glycosyltransferases and other proteins involved in biosynthesis and export of extracellular polysaccharides (EPS), was required for floc formation. One of the two asparagine synthase paralogues, associated with this EPS biosynthesis gene cluster, was required for floc formation in Zoogloea. Similar EPS biosynthesis gene cluster(s) were identified in the genome of other AS proteobacteria including polyphosphate-accumulating Candidatus Accumulibacter phosphatis (CAP) and nitrifying Nitrosopira and Nitrosomonas bacteria, but the gene composition varies interspecifically and intraspecifically. Our results indicate that floc formation of desired AS bacteria, including CAP strains, facilitate their recruitment into AS and gradual enrichment via repeated AS settling and recycling processes. (C) 2016 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Engineering, Environmental ; Environmental Sciences ; Water Resources
研究领域[WOS]	Engineering ; Environmental Sciences & Ecology ; Water Resources
关键词[WOS]	WASTE-WATER TREATMENT ; POLYPHOSPHATE-ACCUMULATING ORGANISMS ; BIOLOGICAL PHOSPHORUS REMOVAL ; ASPARAGINE SYNTHETASE-B ; SEWAGE-TREATMENT PLANTS ; ESCHERICHIA-COLI ; SHEWANELLA-ONEIDENSIS ; SP NOV. ; PHYLOGENETIC ANALYSIS ; COMMUNITY COMPOSITION
收录类别	SCI
语种	英语
WOS记录号	WOS:000381950400049
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28380]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_学位论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Xiamen Univ, Sch Life Sci, Xiamen 361005, Peoples R China 4.Chinese Acad Sci, Inst Genet & Dev, Beijing 100101, Peoples R China 5.Hubei Engn Univ, Sch Life Sci & Technol, Xiaogan 43200, Peoples R China 6.Univ Hong Kong, Dept Civil Engn, Environm Biotechnol Lab, Pokfulam, Hong Kong, Peoples R China 7.Chinese Acad Sci, Inst Deep Sea Sci & Technol, Sanya 572000, Peoples R China
推荐引用方式 GB/T 7714	An, Weixing,Guo, Feng,Song, Yulong,et al. Comparative genomics analyses on EPS biosynthesis genes required for floc formation of Zoogloea resiniphila and other activated sludge bacteria[J]. WATER RESEARCH,2016,102:494-504.
APA	An, Weixing.,Guo, Feng.,Song, Yulong.,Gao, Na.,Bai, Shijie.,...&Qiu, Dongru.(2016).Comparative genomics analyses on EPS biosynthesis genes required for floc formation of Zoogloea resiniphila and other activated sludge bacteria.WATER RESEARCH,102,494-504.
MLA	An, Weixing,et al."Comparative genomics analyses on EPS biosynthesis genes required for floc formation of Zoogloea resiniphila and other activated sludge bacteria".WATER RESEARCH 102(2016):494-504.