Effects of submerged macrophyte Ceratophyllum demersum on ROS generation and cell damage in Microcystis aeruginosa

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	Effects of submerged macrophyte Ceratophyllum demersum on ROS generation and cell damage in Microcystis aeruginosa
	He, Y.1,2; Cheng, L.1; Tian, Y.1; Chen, Z. L.1; Zhou, Q. H.1; Zhang, Y. Y.1; Liu, B. Y.1; Wu, Z. B.1
刊名	ALLELOPATHY JOURNAL
	2016-07-01
卷号	38 期号:2 页码:229-238
关键词	Allelopathic effects biomass cell damage cell membrane Ceratophyllum demersum compounds co-culture Microcystis aeruginosa pheniloc ROS
ISSN号	0971-4693
英文摘要	In laboratory bioassay, we investigated the generation of reactive oxygen species (ROS) in cyanobacteria Microcystis aeruginosa induced by allelopathic effects of submerged macrophyte Ceratophyllum demersum in co-culture system and the changes in cell membrane integrity and DNA damage. We found that the growth inhibition of M. aeruginosa significantly increased with the increase in C. demersum biomass (0.5, 1.0, 3.0 and 6.0 g FW/L). The concentrations of total phenolic compounds (TPCs) secreted by C. demersum in co-culture water increased with the increase in C. demersum biomass and co-culture time. Compared with control, the level of ROS increased in a dose-dependent manner with the biomass of C. demersum and the concentration of TPCs in co-culture water from the second day. According to the propidium iodide (PI) staining results, numbers of cells losing the membrane integrity increased with ROS generation in M. aeruginosa co-cultured with 1.0, 3.0 and 6.0 g FW/L C. demersum on the fourth day. The significant DNA fragmentation was observed in M. aeruginosa cells at 6 g FW/L C. demersum group. These results suggested that the overproduction of ROS induced by the submerged macrophyte C. demersum was the major factor, resulting in the loss of cell membrane integrity and DNA fragmentation and finally leading to the death of M. aeruginosa cells.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Agronomy
研究领域[WOS]	Agriculture
关键词[WOS]	OXIDATIVE STRESS ; GROWTH-INHIBITION ; MYRIOPHYLLUM-SPICATUM ; GREEN-ALGAE ; MECHANISMS ; ALLELOCHEMICALS ; CYANOBACTERIA ; ANTIOXIDANT ; RESPONSES ; EXTRACTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000383470300009
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28365]
专题	水生生物研究所_淡水生态学研究中心_学位论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	He, Y.,Cheng, L.,Tian, Y.,et al. Effects of submerged macrophyte Ceratophyllum demersum on ROS generation and cell damage in Microcystis aeruginosa[J]. ALLELOPATHY JOURNAL,2016,38(2):229-238.
APA	He, Y..,Cheng, L..,Tian, Y..,Chen, Z. L..,Zhou, Q. H..,...&Wu, Z. B..(2016).Effects of submerged macrophyte Ceratophyllum demersum on ROS generation and cell damage in Microcystis aeruginosa.ALLELOPATHY JOURNAL,38(2),229-238.
MLA	He, Y.,et al."Effects of submerged macrophyte Ceratophyllum demersum on ROS generation and cell damage in Microcystis aeruginosa".ALLELOPATHY JOURNAL 38.2(2016):229-238.