The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation

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	The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation
	Hu, Yong-hua ; Sun, Li
刊名	JOURNAL OF PROTEOMICS
	2016-05-17
卷号	140 页码:100-110
关键词	Edwardsiella tarda Fur Virulence Stress Proteomics
英文摘要	Ferric uptake regulator (Fur) is an important transcriptional regulator of Gram-negative bacteria. Edwardsiella tarda is a severe fish bacterial pathogen with a broad host range that includes humans. In this study, we examined the regulatory function of Fur in E. tarda via a proteomic approach. Compared to the wild type TX01, the fur mutant TX01 Delta fur exhibited (i) retarded growth, (ii) enhanced siderophore production, (iii) increased acid tolerance, which is in contrast to observations in other bacterial species, (iv) decreased survival against oxidative stress and host serum, (v) impaired ability to inhibit host immune response, (vi) attenuated tissue infectivity and overall virulence. The deficiency of TX01 Delta fur was rescued by introduction of an exogenous fur gene. iTRAQ-based comparative proteomic analysis of TX01 Delta fur and TX01 identified 89 differentially expressed proteins that cover a wide range of functional categories including those affected by fur mutation. In addition, 16 proteins were identified for the first time to be regulated by Fur in Gram-negative bacteria. These results provide the first protein based interpretation of the global impact of Fur on the physiology and infectivity of E. tarda.
收录类别	SCI
语种	英语
WOS记录号	WOS:000376697200009
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/131086]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yong-hua,Sun, Li. The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation[J]. JOURNAL OF PROTEOMICS,2016,140:100-110.
APA	Hu, Yong-hua,&Sun, Li.(2016).The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation.JOURNAL OF PROTEOMICS,140,100-110.
MLA	Hu, Yong-hua,et al."The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation".JOURNAL OF PROTEOMICS 140(2016):100-110.