Fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	Fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics
	Kumar, Yashwant 1; Zhang, Limin 2; Panigrahi, Priyabrata 1; Dholakia, Bhushan B.1; Dewangan, Veena 1; Chavan, Sachin G.1; Kunjir, Shrikant M.3; Wu, Xiangyu 2; Li, Ning 2; Rajmohanan, Pattuparambil R.3
刊名	PLANT BIOTECHNOLOGY JOURNAL
	2016-07-01
卷号	14 期号:7 页码:1589-1603
关键词	Chickpea Fusarium oxysporum plant-pathogen interaction proteomics metabolomics NMR
英文摘要	Molecular changes elicited by plants in response to fungal attack and how this affects plant-pathogen interaction, including susceptibility or resistance, remain elusive. We studied the dynamics in root metabolism during compatible and incompatible interactions between chickpea and Fusarium oxysporum f. sp. ciceri (Foc), using quantitative label-free proteomics and NMR-based metabolomics. Results demonstrated differential expression of proteins and metabolites upon Foc inoculations in the resistant plants compared with the susceptible ones. Additionally, expression analysis of candidate genes supported the proteomic and metabolic variations in the chickpea roots upon Foc inoculation. In particular, we found that the resistant plants revealed significant increase in the carbon and nitrogen metabolism; generation of reactive oxygen species (ROS), lignification and phytoalexins. The levels of some of the pathogenesis-related proteins were significantly higher upon Foc inoculation in the resistant plant. Interestingly, results also exhibited the crucial role of altered Yang cycle, which contributed in different methylation reactions and unfolded protein response in the chickpea roots against Foc. Overall, the observed modulations in the metabolic flux as outcome of several orchestrated molecular events are determinant of plant's role in chickpea-Foc interactions.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Plant Sciences
研究领域[WOS]	Biotechnology & Applied Microbiology ; Plant Sciences
关键词[WOS]	F-SP CICERI ; COPPER AMINE OXIDASE ; MASS-SPECTROMETRY ; DEFENSE RESPONSES ; GENE-EXPRESSION ; DIFFERENTIAL MODULATION ; MEDICAGO-TRUNCATULA ; DISEASE RESISTANCE ; PATHOGEN INFECTION ; TRITICUM-AESTIVUM
收录类别	SCI
语种	英语
WOS记录号	WOS:000378741200010
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/9414]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.CSIR Natl Chem Lab, Div Biochem Sci, Pune, Maharashtra, India 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, Key Lab Magnet Resonance Biol Syst, Wuhan, Peoples R China 3.CSIR Natl Chem Lab, Cent NMR Facil, Pune, Maharashtra, India 4.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Metabol & Syst Biol Lab, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Kumar, Yashwant,Zhang, Limin,Panigrahi, Priyabrata,et al. Fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics[J]. PLANT BIOTECHNOLOGY JOURNAL,2016,14(7):1589-1603.
APA	Kumar, Yashwant.,Zhang, Limin.,Panigrahi, Priyabrata.,Dholakia, Bhushan B..,Dewangan, Veena.,...&Gupta, Vidya S..(2016).Fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics.PLANT BIOTECHNOLOGY JOURNAL,14(7),1589-1603.
MLA	Kumar, Yashwant,et al."Fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics".PLANT BIOTECHNOLOGY JOURNAL 14.7(2016):1589-1603.