REE bound DNA in natural plant

	REE bound DNA in natural plant
	Wang YQ(王玉琦); Jiang P(江平); Guo FQ(郭繁清); Zhang ZY(张智勇); Sun JX(孙景信); Wang, YQ; Jiang, P; Guo, FQ; Zhang, ZY; Sun, JX
刊名	SCIENCE IN CHINA SERIES B-CHEMISTRY
	1999
卷号	42 期号:4 页码:357-362
关键词	nucleic acids DNA REE MAA INAA
通讯作者	Chinese Acad Sci, Inst High Energy Phys, Lab Nucl Anal Techniques, Beijing 100080, Peoples R China ; Chinese Acad Agr, Grad Sch, Beijing 100081, Peoples R China
英文摘要	The binding of rare earth elements (REEs) with nucleic acids in the leaves of fern Dicranopteris dt chotoma (DD) has been studied by molecular activation analysis (MAA). The REEs bound DNA (REE-DNA) was obtained from the leaves of DD. The CTAB-based procedure was modified for extraction of total DNA. The purity of DNA was examined by UV spectroscopy. The DNA obtained was separated and determined by agarose gel electrophoresis further. Meanwhile, the contents of eight rare earth elements (La,ee,Nd,Sm,Eu,Tb,Yb and Lu) in REE-DNA were detected by instrumental neutron activation analysis (INAA). The results showed that REE-DNA with higher purity could be extracted from plant using this method. It was also found that REEs were bound firmly with DNA in the leaves of DD. The molecular weight (MW) of REE-DNA band was about 22 kb in agarose gel electrophoresis.
学科主题	Chemistry
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
原文出处	SCI
语种	英语
WOS记录号	WOS:000082227600004
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/237134]
专题	高能物理研究所_多学科研究中心高能物理研究所_实验物理中心高能物理研究所_加速器中心中国科学院高能物理研究所_中国散裂中子源
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Wang YQ,Jiang P,Guo FQ,et al. REE bound DNA in natural plant[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,1999,42(4):357-362.
APA	王玉琦.,江平.,郭繁清.,张智勇.,孙景信.,...&Cao, GY.(1999).REE bound DNA in natural plant.SCIENCE IN CHINA SERIES B-CHEMISTRY,42(4),357-362.
MLA	王玉琦,et al."REE bound DNA in natural plant".SCIENCE IN CHINA SERIES B-CHEMISTRY 42.4(1999):357-362.