Vertically Scanned Laser Sheet Microscopy

CORC > 自动化研究所 > 中国科学院自动化研究所 > 中国科学院分子影像重点实验室

	Vertically Scanned Laser Sheet Microscopy
	Di Dong 1; Alicia Arranz 2; ShouPing Zhu 3; Yujie Yang 1; Liangliang Shi 1; Jun Wang 4; Chen Shen 3; Jie Tian 1; Jorge Ripoll 5,6,7; Dong, Di
刊名	Journal of Biomedical Optics
	2014-10
卷号	19 期号:10 页码:106001-8
关键词	light sheet microscopy selective plane illumination microscopy stripe artifact
通讯作者	Jie Tian, Jorge Ripoll
英文摘要	Laser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering structures are very common, greatly affecting image quality. To solve this problem, we report here a two-step procedure which consists of continuously acquiring laser sheet images while vertically displacing the sample, and then using the variational stationary noise remover (VSNR) method to further reduce the remaining stripes. Images from a cleared murine colon acquired with a vertical scan are compared with common stitching procedures demonstrating that vertically scanned light sheet microscopy greatly improves the performance of current light sheet microscopy approaches without the need for complex changes to the imaging setup and allows imaging of elongated samples, extending the field of view in the vertical direction.
收录类别	SCI
语种	英语
WOS记录号	WOS:000345837200018
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/11158]
专题	自动化研究所_中国科学院分子影像重点实验室
作者单位	1.aChinese Academy of Sciences, Key Laboratory of Molecular Imaging, Beijing 100190, China 2.Swiss Federal Institute of Technology (ETH-Zurich), Institute for Biomedical Engineering, Switzerland 3.Xidian University, Xian, School of Life Science and Technology, Shaanxi 710071, China 4.Harbin University of Science and Technology, School of Automation, Harbin 150080, China 5.Universidad Carlos III of Madrid, Department of Bioengineering and Aerospace Engineering, Madrid 28911, Spain 6.Instituto de Investigación Sanitaria del Hospital Gregorio Marañón, Experimental Medicine and Surgery Unit, Madrid 28007, Spain 7.Foundation for Research and Technology-Hellas, Institute of Electronic Structure and Laser, Heraklion 71110, Greece
推荐引用方式 GB/T 7714	Di Dong,Alicia Arranz,ShouPing Zhu,et al. Vertically Scanned Laser Sheet Microscopy[J]. Journal of Biomedical Optics,2014,19(10):106001-8.
APA	Di Dong.,Alicia Arranz.,ShouPing Zhu.,Yujie Yang.,Liangliang Shi.,...&Tian, Jie.(2014).Vertically Scanned Laser Sheet Microscopy.Journal of Biomedical Optics,19(10),106001-8.
MLA	Di Dong,et al."Vertically Scanned Laser Sheet Microscopy".Journal of Biomedical Optics 19.10(2014):106001-8.