Illuminating biological processes through site-specific protein labeling

	Illuminating biological processes through site-specific protein labeling
	Zhang, Gong 1,2; Zheng, Siqi 3; Liu, Haiping 3; Chen, Peng R.2,3
刊名	chemical society reviews
	2015
卷号	44 期号:11 页码:3405-3417
英文摘要	coupling genetically encoded peptide tags or unnatural amino acids (uaas) with bioorthogonal reactions allows for precise control over the protein-labeling sites as well as the wide choice of labeling dyes. however, the value of these site-specific protein labeling strategies in a real biology setting, particularly their advantages over conventional labeling methods including fluorescent proteins (fps), remains to be fully demonstrated. in this tutorial review, we first introduce various strategies for site-specific protein labeling that utilize artificial peptide sequences or genetically encoded uaas as the labeling handle. emphasis will be placed on introducing the advantages of protein site-specific labeling techniques as well as their applications in solving biological problems, particularly as to why a site-specific protein labeling approach is needed. finally, beyond the widely used single site-specific labeling methods, the recently emerged dual site-specific protein labeling strategies will be introduced together with their fast-growing potential in illustrating biological processes.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	unnatural amino-acids ; cell-surface proteins ; genetic-code ; mammalian-cells ; living cells ; chemistry ; fluorescence ; activation ; microscopy ; fluorophore
收录类别	SCI
语种	英语
WOS记录号	WOS:000355485900004
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/146303]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China 2.Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China 3.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Synthet & Funct Biomol Ctr,Coll Chem, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Gong,Zheng, Siqi,Liu, Haiping,et al. Illuminating biological processes through site-specific protein labeling[J]. chemical society reviews,2015,44(11):3405-3417.
APA	Zhang, Gong,Zheng, Siqi,Liu, Haiping,&Chen, Peng R..(2015).Illuminating biological processes through site-specific protein labeling.chemical society reviews,44(11),3405-3417.
MLA	Zhang, Gong,et al."Illuminating biological processes through site-specific protein labeling".chemical society reviews 44.11(2015):3405-3417.