Dynamics of the Lipid Droplet Proteome of the Oleaginous Yeast Rhodosporidium toruloides

	Dynamics of the Lipid Droplet Proteome of the Oleaginous Yeast Rhodosporidium toruloides
	Zhu, Zhiwei 1; Ding, Yunfeng 2; Gong, Zhiwei 1; Yang, Li 2; Zhang, Sufang 1,3; Zhang, Congyan 2; Lin, Xinping 1; Shen, Hongwei 1,3; Zou, Hanfa 1; Xie, Zhensheng 2
刊名	eukaryotic cell
	2015-03-01
卷号	14 期号:3 页码:252-264
英文摘要	lipid droplets (lds) are ubiquitous organelles that serve as a neutral lipid reservoir and a hub for lipid metabolism. manipulating ld formation, evolution, and mobilization in oleaginous species may lead to the production of fatty acid-derived biofuels and chemicals. however, key factors regulating ld dynamics remain poorly characterized. here we purified the lds and identified ld-associated proteins from cells of the lipid-producing yeast rhodosporidium toruloides cultured under nutrient-rich, nitrogen-limited, and phosphorus-limited conditions. the ld proteome consisted of 226 proteins, many of which are involved in lipid metabolism and ld formation and evolution. further analysis of our previous comparative transcriptome and proteome data sets indicated that the transcription level of 85 genes and protein abundance of 77 proteins changed under nutrient-limited conditions. such changes were highly relevant to lipid accumulation and partially confirmed by reverse transcription-quantitative pcr. we demonstrated that the major ld structure protein ldp1 is an ld marker protein being upregulated in lipid-rich cells. when overexpressed in saccharomyces cerevisiae, ldp1 localized on the ld surface and facilitated giant ld formation, suggesting that ldp1 plays an important role in controlling ld dynamics. our results significantly advance the understanding of the molecular basis of lipid overproduction and storage in oleaginous yeasts and will be valuable for the development of superior lipid producers.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	microbiology ; mycology
研究领域[WOS]	microbiology ; mycology
关键词[WOS]	saccharomyces-cerevisiae ; aspergillus-nidulans ; biodiesel production ; high confidence ; body protein ; fat storage ; reveals ; identification ; localization ; accumulation
收录类别	SCI
语种	英语
WOS记录号	WOS:000350666000006
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/146069]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Zhiwei,Ding, Yunfeng,Gong, Zhiwei,et al. Dynamics of the Lipid Droplet Proteome of the Oleaginous Yeast Rhodosporidium toruloides[J]. eukaryotic cell,2015,14(3):252-264.
APA	Zhu, Zhiwei.,Ding, Yunfeng.,Gong, Zhiwei.,Yang, Li.,Zhang, Sufang.,...&Zhao, Zongbao K..(2015).Dynamics of the Lipid Droplet Proteome of the Oleaginous Yeast Rhodosporidium toruloides.eukaryotic cell,14(3),252-264.
MLA	Zhu, Zhiwei,et al."Dynamics of the Lipid Droplet Proteome of the Oleaginous Yeast Rhodosporidium toruloides".eukaryotic cell 14.3(2015):252-264.