The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes

	The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes
	Meng, Yingying 1,2; Jiang, Junpeng 1,3; Wang, Haitao 1,3; Cao, Xupeng 1; Xue, Song 1; Yang, Qing 2; Wang, Weiliang 4
刊名	bioresource technology
	2015-03-01
卷号	179 页码:483-489
关键词	Nannochloropsis oceanica IMET1 TAG EPA Nitrogen limitation Fatty acid composition
英文摘要	the strategy of nitrogen limitation has been widely applied to enhance lipid production in microalgae. the changes of cellular composition, and the characteristics of triacylglycerol (tag) and eicosapentaenoic acid (epa) accumulation in nannochloropsis oceanica imet1 were investigated. the results revealed that after nitrogen limitation tag rather than carbohydrate was the dominant carbon sink in n. oceanica imet1. different nitrogen supplementation strategies were applied in order to achieve high tag and epa productivity, respectively. limited nitrogen was supplied to improve tag production, and a maximum productivity of 29.44 mg l-1 d(-1) was obtained, which was a 6.74-fold increase compared to nitrogen-depleted cultivation. the highest epa productivity of 7.66 mg l-1 d(-1) was achieved under nitrogenreplete cultivation, which is different from the condition for tag maximum productivity because the epa is in glycolipids and phospholipids mainly. the fatty acid composition analysis identified the source of acyl group in tag accumulation. (c) 2014 elsevier ltd. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; technology
类目[WOS]	agricultural engineering ; biotechnology & applied microbiology ; energy & fuels
研究领域[WOS]	agriculture ; biotechnology & applied microbiology ; energy & fuels
关键词[WOS]	chlamydomonas-reinhardtii ; triacylglycerol accumulation ; lipid production ; microalgae ; starvation ; biodiesel ; optimization ; protein ; growth
收录类别	SCI
语种	英语
WOS记录号	WOS:000348041600064
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145955]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
推荐引用方式 GB/T 7714	Meng, Yingying,Jiang, Junpeng,Wang, Haitao,et al. The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes[J]. bioresource technology,2015,179:483-489.
APA	Meng, Yingying.,Jiang, Junpeng.,Wang, Haitao.,Cao, Xupeng.,Xue, Song.,...&Wang, Weiliang.(2015).The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes.bioresource technology,179,483-489.
MLA	Meng, Yingying,et al."The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes".bioresource technology 179(2015):483-489.