Development of Urinary Pseudotargeted LC-MS-Based Metabolomics Method and Its Application in Hepatocellular Carcinoma Biomarker Discovery

	Development of Urinary Pseudotargeted LC-MS-Based Metabolomics Method and Its Application in Hepatocellular Carcinoma Biomarker Discovery
	Shao, Yaping 1; Zhu, Bin 2; Zheng, Ruiyin 3; Zhao, Xinjie 1; Yin, Peiyuan 1; Lu, Xin 1; Jiao, Binghua 3; Xu, Guowang 1; Yao, Zhenzhen 3
刊名	journal of proteome research
	2015-02-01
卷号	14 期号:2 页码:906-916
关键词	pseudotargeted method LC-MS metabolomics urine cirrhosis hepatocellular carcinoma
英文摘要	hepatocellular carcinoma (hcc) is one of the pestilent malignancies leading to cancer-related death. discovering effective biomarkers for hcc diagnosis is an urgent demand. to identify potential metabolite biomarkers, we developed a urinary pseudotargeted method based on liquid chromatography-hybrid triple quadrupole linear ion trap mass spectrometry (lc-qtrap ms). compared with nontargeted method, the pseudotargeted method can achieve better data quality, which benefits differential metabolites discovery. the established method was applied to cirrhosis (cir) and hcc investigation. it was found that urinary nucleosides, bile acids, citric acid, and several amino acids were significantly changed in liver disease groups compared with the controls, featuring the dysregulation of purine metabolism, energy metabolism, and amino metabolism in liver diseases. furthermore, some metabolites such as cyclic adenosine monophosphate, glutamine, and short- and medium-chain acylcarnitines were the differential metabolites of hcc and cir. on the basis of binary logistic regression, butyrylcarnitine (carnitine c4:0) and hydantoin-5-propionic acid were defined as combinational markers to distinguish hcc from cir. the area under curve was 0.786 and 0.773 for discovery stage and validation stage samples, respectively. these data show that the established pseudotargeted method is a complementary one of targeted and nontargeted methods for metabolomics study.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	chromatography-mass spectrometry ; liver-cirrhosis ; serum metabolomics ; tumor-markers ; hepatitis-b ; cancer ; metabolism ; nucleosides ; diagnosis ; acylcarnitines
收录类别	SCI
语种	英语
WOS记录号	WOS:000349276400030
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145947]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Dept Biliary Surg 2, Shanghai 200438, Peoples R China 3.Second Mil Med Univ, Dept Biochem & Mol Biol, Shanghai 200433, Peoples R China
推荐引用方式 GB/T 7714	Shao, Yaping,Zhu, Bin,Zheng, Ruiyin,et al. Development of Urinary Pseudotargeted LC-MS-Based Metabolomics Method and Its Application in Hepatocellular Carcinoma Biomarker Discovery[J]. journal of proteome research,2015,14(2):906-916.
APA	Shao, Yaping.,Zhu, Bin.,Zheng, Ruiyin.,Zhao, Xinjie.,Yin, Peiyuan.,...&Yao, Zhenzhen.(2015).Development of Urinary Pseudotargeted LC-MS-Based Metabolomics Method and Its Application in Hepatocellular Carcinoma Biomarker Discovery.journal of proteome research,14(2),906-916.
MLA	Shao, Yaping,et al."Development of Urinary Pseudotargeted LC-MS-Based Metabolomics Method and Its Application in Hepatocellular Carcinoma Biomarker Discovery".journal of proteome research 14.2(2015):906-916.