Study of metabolite differences of flue-cured tobacco from different regions using a pseudotargeted gas chromatography with mass spectrometry selected-ion monitoring method

	Study of metabolite differences of flue-cured tobacco from different regions using a pseudotargeted gas chromatography with mass spectrometry selected-ion monitoring method
	Zhao, Yanni; Zhao, Chunxia; Li, Yanli; Chang, Yuwei; Zhang, Junjie; Zeng, Zhongda; Lu, Xin; Xu, Guowang
刊名	journal of separation science
	2014-08-01
卷号	37 期号:16 页码:2177-2184
关键词	Flue-cured tobacco Gas chromatography with mass spectrometry Pseudotargeted analysis Selected-ion monitoring
英文摘要	a pseudotargeted method based on gas chromatography and mass spectrometry with selected-ion monitoring was established to investigate the metabolite differences of flue-cured tobacco from three different growing regions. the mixed solvent of acetonitrile/isopropanol/water (3:3:2, v/v/v) was chosen as the optimal extraction system based on the good repeatability and extraction efficiency. a self-developed software coupled with commercial software was used to establish the pseudotargeted method including 289 peaks and 47 groups. multivariable statistical analysis indicated that tobacco samples can be obviously separated based on the geographical origins. on the basis of a mann-whitney u test, organic acids, phenols, and alkaloids had higher levels in hunan province. in contrast, a large proportion of amino acids (including l-tyrosine, l-proline, and serine), sucrose, and linoleic acid were the highest in yunnan province. meanwhile, multiple metabolic pathways (including carbohydrate metabolism, tricarboxylic acid cycle, and nitrogen metabolism) were influenced by growing regions. twenty-eight differential metabolites, which had great contributions to the classification of tobacco samples of three growing regions, were further defined. the results demonstrated that the developed pseudotargeted method was a powerful tool to investigate the metabolic profiling of tobacco leaves and discriminate tobacco leaves of different growing regions.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	performance liquid-chromatography ; volatile organic-acids ; gc-ms ; transgenic rice ; quality-control ; wild-type ; reveals ; leaves ; arabidopsis ; responses
收录类别	SCI
语种	英语
WOS记录号	WOS:000340556300012
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145840]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Yanni,Zhao, Chunxia,Li, Yanli,et al. Study of metabolite differences of flue-cured tobacco from different regions using a pseudotargeted gas chromatography with mass spectrometry selected-ion monitoring method[J]. journal of separation science,2014,37(16):2177-2184.
APA	Zhao, Yanni.,Zhao, Chunxia.,Li, Yanli.,Chang, Yuwei.,Zhang, Junjie.,...&Xu, Guowang.(2014).Study of metabolite differences of flue-cured tobacco from different regions using a pseudotargeted gas chromatography with mass spectrometry selected-ion monitoring method.journal of separation science,37(16),2177-2184.
MLA	Zhao, Yanni,et al."Study of metabolite differences of flue-cured tobacco from different regions using a pseudotargeted gas chromatography with mass spectrometry selected-ion monitoring method".journal of separation science 37.16(2014):2177-2184.