A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells

	A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells
	Liu, Zhao-Ming 1,4; Feng, Lei 2; Hou, Jie 3; Lv, Xia 1; Ning, Jing 1,3; Ge, Guang-Bo 1; Wang, Ke-Wei 2; Cui, Jing-Nan 2; Yang, Ling 1
刊名	sensors and actuators b-chemical
	2014-12-15
卷号	205 页码:151-157
关键词	Human carboxylesterase 2 Ratiometric fluorescent probe 4-Hydroxy-N-butyl-1 Bioimaging 8-naphthalimide
英文摘要	a new ratiometric fluorescent probe derived from 4-hydroxy-n-butyl-1,8-naphthalimide (hnn) has been developed for selective detection of human carboxylesterase 2 (hce2). the probe is designed by introducing benzoyl moiety to hnn, based on the intramolecular charge transfer (ict) mechanism. the probe displays satisfying stability under physiological ph conditions with very low background fluorescence signal, but it can be rapidly hydrolyzed by hce2 and release of hnn which leads to a remarkable red shift in emission spectra (148 nm). the newly designed probe exhibits excellent selectivity towards hce2 over other human hydrolases, while the interference from various biologically relevant chemicals can be negligible. its potential biological applications including inhibitor screening using human tissue preparations as enzyme sources, as well as fluorescence imaging of endogenous hce2 in human living cells, have also been demonstrated. (c) 2014 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences ; technology
类目[WOS]	chemistry, analytical ; electrochemistry ; instruments & instrumentation
研究领域[WOS]	chemistry ; electrochemistry ; instruments & instrumentation
关键词[WOS]	human intestinal carboxylesterase ; mammalian carboxylesterases ; human liver ; catalytic-properties ; cpt-11 hydrolysis ; tumor-tissue ; probe ; irinotecan ; inhibitors ; metabolism
收录类别	SCI
语种	英语
WOS记录号	WOS:000343117600020
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145788]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116024, Peoples R China 2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China 3.Dalian Med Univ, Dalian 116044, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhao-Ming,Feng, Lei,Hou, Jie,et al. A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells[J]. sensors and actuators b-chemical,2014,205:151-157.
APA	Liu, Zhao-Ming.,Feng, Lei.,Hou, Jie.,Lv, Xia.,Ning, Jing.,...&Yang, Ling.(2014).A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells.sensors and actuators b-chemical,205,151-157.
MLA	Liu, Zhao-Ming,et al."A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells".sensors and actuators b-chemical 205(2014):151-157.