Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions

	Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions
	Meng, Yingying 1,2; Yao, Changhong 1,3; Xue, Song 1; Yang, Haibo 2
刊名	bioresource technology
	2014
卷号	151 页码:347-354
关键词	FT-IR Microalgae Cell composition Strain screening Physiological status
英文摘要	fourier transform infrared spectroscopy (ft-ir) was applied in algal strain screening and monitoring cell composition dynamics in a marine microalga isochrysis zhangjiangensis during algal cultivation. the content of lipid, carbohydrate and protein of samples determined by traditional methods had validated the accuracy of ft-ir method. for algal screening, the band absorption ratios of lipid/amide i and carbo/amide i from ft-ir measurements allowed for the selection of isochrysis sp. and tetraselmis subcordiformis as the most potential lipid and carbohydrate producers, respectively. the cell composition dynamics oft zhangjiangensis measured by ft-ir revealed the diversion of carbon allocation from protein to carbohydrate and neutral lipid when nitrogen-replete cells were subjected to nitrogen limitation. the carbo/amide i band absorption ratio had also been demonstrated to depict physiological status under nutrient stress in t. subcordiformis. ft-ir serves as a tool for the simultaneous measurement of lipid, carbohydrate, and protein content in cell. (c) 2013 elsevier ltd. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; technology
类目[WOS]	agricultural engineering ; biotechnology & applied microbiology ; energy & fuels
研究领域[WOS]	agriculture ; biotechnology & applied microbiology ; energy & fuels
关键词[WOS]	biomass composition ; chlamydomonas-reinhardtii ; carbon allocation ; algal biomass ; spectra ; microspectroscopy ; phytoplankton ; accumulation ; protein ; starch
收录类别	SCI
语种	英语
WOS记录号	WOS:000330085800048
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145512]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Meng, Yingying,Yao, Changhong,Xue, Song,et al. Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions[J]. bioresource technology,2014,151:347-354.
APA	Meng, Yingying,Yao, Changhong,Xue, Song,&Yang, Haibo.(2014).Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions.bioresource technology,151,347-354.
MLA	Meng, Yingying,et al."Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions".bioresource technology 151(2014):347-354.