Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS

	Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS
	Deng, Zhenzhen 1,2; Ye, Mingliang 1; Bian, Yangyang 1,2; Liu, Zheyi 1,2; Liu, Fangjie 1,2; Wang, Chunli 1,2; Qin, Hongqiang 1; Zou, Hanfa 1
刊名	chemical communications
	2014
卷号	50 期号:90 页码:13960-13962
通讯作者	叶明亮 ; 邹汉法
英文摘要	a simple, cost-effective and high throughput method was developed for multiplexed kinase activity assay based on the multiplex isotope labeling of designed substrate peptides. this strategy was successfully applied to monitor the time-dependent consumption of substrates and generation of products in the single and multiple substrate systems.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	protein-kinase ; proteomics ; cancer
收录类别	SCI
语种	英语
WOS记录号	WOS:000343985600022
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/144507]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Deng, Zhenzhen,Ye, Mingliang,Bian, Yangyang,et al. Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS[J]. chemical communications,2014,50(90):13960-13962.
APA	Deng, Zhenzhen.,Ye, Mingliang.,Bian, Yangyang.,Liu, Zheyi.,Liu, Fangjie.,...&Zou, Hanfa.(2014).Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS.chemical communications,50(90),13960-13962.
MLA	Deng, Zhenzhen,et al."Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS".chemical communications 50.90(2014):13960-13962.