A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis

	A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis
	Chang, Yuwei 1; Zhang, Lei 2; Lu, Xin 1; Zhao, Chunxia 1; Zhu, Zhen 2; Wang, Feng 3; Zhang, Junjie 1; Chen, Shili 1; Zhao, Yanni 1; Xu, Guowang 1
刊名	metabolomics
	2014-12-01
卷号	10 期号:6 页码:1197-1209
关键词	Methyl tert-butyl ether Metabolomics Lipidomics Insecticide treatment Transgenic rice
通讯作者	许国旺
英文摘要	abiotic stress caused by insecticide treatment is an interesting and challenging topic in plant research. here a simultaneous extraction method with methyl tert-butyl ether for metabolomics and lipidomics analysis by using rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry was developed and validated. the extraction efficiency of lipidome and metabolome based on the current developed method showed an obvious improvement compared with literatures. about 300 metabolites were identified in rice leaf. method validation was performed and analytical properties including the linearity (r-2, 0.991-1.000), repeatability (over 87 % peaks with cv < 20 % accounting for over 92 % of total response) and recovery (74.4-119.6 %) were satisfactory. the method was then applied to investigate time-course changes caused by insecticide treatment in transgenic rice with cry1ac and sck genes and its wild counterpart. antioxidants including ferulic acid and sinapic acid were down-regulated at 24 h after insecticide treatment. signaling metabolites including salicylic acid and nicotinamide adenine dinucleotide were significantly up-regulated at 12-24 h after treatment in transgenic rice. more flavonoids were significantly changed in transgenic plants. results indicated that gene transformation affected the metabolic response of rice to insecticide stress.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	endocrinology & metabolism
研究领域[WOS]	endocrinology & metabolism
关键词[WOS]	performance liquid-chromatography ; tert-butyl ether ; mass-spectrometry ; metabonomic analysis ; hplc-ms ; plants ; flavonoids ; leaves ; stress ; acid
收录类别	SCI
语种	英语
WOS记录号	WOS:000344555300016
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/144197]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 3.Fujian Acad Agr Sci, Fujian Prov Key Lab Genet Engn Agr, Fuzhou 350003, Peoples R China
推荐引用方式 GB/T 7714	Chang, Yuwei,Zhang, Lei,Lu, Xin,et al. A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis[J]. metabolomics,2014,10(6):1197-1209.
APA	Chang, Yuwei.,Zhang, Lei.,Lu, Xin.,Zhao, Chunxia.,Zhu, Zhen.,...&Xu, Guowang.(2014).A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis.metabolomics,10(6),1197-1209.
MLA	Chang, Yuwei,et al."A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis".metabolomics 10.6(2014):1197-1209.