Polyaniline sheathed electrospun nanofiber bar for in vivo extraction of trace acidic phytohormones in plant tissue

	Polyaniline sheathed electrospun nanofiber bar for in vivo extraction of trace acidic phytohormones in plant tissue
	Wu, Qian 1,2; Wu, Dapeng 1; Guan, Yafeng 1
刊名	journal of chromatography a
	2014-05-16
卷号	1342 期号:1 页码:16-23
关键词	In vivo solid-phase micro-extraction Polyaniline Electrospun nanofibers Phytohormones Aloe leaf
通讯作者	吴大朋 ; 关亚风
英文摘要	for in vivo extraction of trace and polar components in tissues, extraction materials should be biocompatible, highly permeable to matrix and have high extraction capacity for polar and ionic compounds. in this study, nanofibers (similar to 200 nm) with rough polyaniline (pan) sheath were prepared successfully for the first time by coaxial electrospinning and coelectrospun polymer sacrificing method. the nanofibers (0.5 mg) were then compressed into tiny bar (phi 1 mm x 2 mm) and inserted directly into the aloe leaf for in vivo extraction of trace phytohormones. due to the large surface area (13.97 m(2)/g) of nanofibers and large through pores formed between fibers, high extraction capacity (up to 10 mu g/bar) and permeability to tissue matrix of the tiny bar can be obtained. moreover, pan sheath has high adsorption equilibrium constants (10(7)-10(8) ml/mol) for acidic phytohormones in aloe leaf. four phytohormones were detected by in vivo extraction with a spatial resolution of 3-8 mm(3), temporal resolution of 20 min, and limit of detection down to 60 pg/g. the quantification results were well met with that by traditional in vitro organic solvent extraction. (c) 2014 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	solid-phase microextraction ; mass-spectrometry ; biological-fluids ; polymer blends ; fibers ; pharmaceuticals ; conductivity ; fish
收录类别	SCI
语种	英语
WOS记录号	WOS:000335804500003
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/144137]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wu, Qian,Wu, Dapeng,Guan, Yafeng. Polyaniline sheathed electrospun nanofiber bar for in vivo extraction of trace acidic phytohormones in plant tissue[J]. journal of chromatography a,2014,1342(1):16-23.
APA	Wu, Qian,Wu, Dapeng,&Guan, Yafeng.(2014).Polyaniline sheathed electrospun nanofiber bar for in vivo extraction of trace acidic phytohormones in plant tissue.journal of chromatography a,1342(1),16-23.
MLA	Wu, Qian,et al."Polyaniline sheathed electrospun nanofiber bar for in vivo extraction of trace acidic phytohormones in plant tissue".journal of chromatography a 1342.1(2014):16-23.