A Fragmentation Study of Six C-21 Steroidal Aglycones by Electrospray Ionization Ion-Trap Time-of-Flight Tandem Mass Spectrometry

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	A Fragmentation Study of Six C-21 Steroidal Aglycones by Electrospray Ionization Ion-Trap Time-of-Flight Tandem Mass Spectrometry
	Chen, Xing-Long 1,2; Geng, Chang-An1 ; Chen, Ji-Jun1
刊名	NATURAL PRODUCT COMMUNICATIONS
	2015-12-01
卷号	10 期号:12 页码:2053-2058
关键词	ESI-IT-TOF-MSn Fragmentation pathways Diagnostic ions C-21 Steroidal aglycones
英文摘要	The fragmentation patterns of six C-21 steroidal aglycones, metaplexigenin (1), caudatin (2), qingyangshengenin (3), penupogenin (4), 20-cinnamoylsarcostin (5), and gagamine (6), were analyzed by high-resolution electrospray ionization ion-trap time-of-flight tandem mass spectrometry (HR-ESI-IT-TOF-MSn). The [M-H](+) ions of steroids 1-3 that contain a carbonyl functional group at C-20 (Type I) and [M+H](+) ions of steroids 5-6 that possess a hydroxyl group at C-20 (Type II) were readily observed in MS analyses. The fragmentation pathways and diagnostic fragment ions for these six steroidal aglycones were proposed on the basis of their MS' analyses. The common fragmentation pathways for type I steroidal aglycones include the neutral loss of the ester group at C-12 and the hydroxyl moieties on the steroid skeleton, as well as the cleavage of ring D. Their diagnostic fragment ions were identified as m/z 361(B), 343 (C), 325 (D), 307 (F), 283 (G), 259 (E), and 243 (H). The fragmentation behavior of penupogenin (4) in type II was similar to those of type I, with m/z 363 (B'), 345 (C'), 327 (D'), 309 (F'), 283 (G), and 243 (H) as its diagnostic fragment ions. The ester group at C-20 was difficult to cleave in the MSn analyses of 20-cinnamoylsarcostin (5) and gagamine (6) so that the loss of this ester group was slower than that at C-12 and hydroxyl groups; the key ions at m/z 329 (I), 311(J), 293 (K), and 275 (L) were characteristic for 5 and 6. The base ion peaks were derived from the loss of the substituent group at either C-12 or C-17 for both type land type II steroidal aglycones.
类目[WOS]	Chemistry, Medicinal ; Food Science & Technology
研究领域[WOS]	Pharmacology & Pharmacy ; Food Science & Technology
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; B VIRUS AGENTS ; CYNANCHUM-CHEKIANGENSE ; IDENTIFICATION ; GLYCOSIDES ; SAPONINS ; CAUDATIN ; ROOTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000367211300013
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/25431]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xing-Long,Geng, Chang-An,Chen, Ji-Jun. A Fragmentation Study of Six C-21 Steroidal Aglycones by Electrospray Ionization Ion-Trap Time-of-Flight Tandem Mass Spectrometry[J]. NATURAL PRODUCT COMMUNICATIONS,2015,10(12):2053-2058.
APA	Chen, Xing-Long,Geng, Chang-An,&Chen, Ji-Jun.(2015).A Fragmentation Study of Six C-21 Steroidal Aglycones by Electrospray Ionization Ion-Trap Time-of-Flight Tandem Mass Spectrometry.NATURAL PRODUCT COMMUNICATIONS,10(12),2053-2058.
MLA	Chen, Xing-Long,et al."A Fragmentation Study of Six C-21 Steroidal Aglycones by Electrospray Ionization Ion-Trap Time-of-Flight Tandem Mass Spectrometry".NATURAL PRODUCT COMMUNICATIONS 10.12(2015):2053-2058.