Engineering DNA Three-Way Junction with Multifunctional Moieties: Sensing Platform for Bioanalysis

	Engineering DNA Three-Way Junction with Multifunctional Moieties: Sensing Platform for Bioanalysis
	Zhang,Libing; Guo,Shaojun; Zhu,Jinbo; Zhou,Zhixue; Li,Tao; Li,Jing; Dong,Shaojun; Wang,Erkang
刊名	analytical chemistry
	2015
卷号	87 期号:22 页码:11295-11300
关键词	NANOCLUSTERS PROTEIN NANOARCHITECTURES NANOPARTICLES NANOARRAYS RNA ATP
通讯作者	dong,sj
英文摘要	functionalization of dna nanostructures is critical to the achievement of the application in biosensors. herein, we demonstrate a novel dna three-way junction (twj) with three functional moieties, which integrates the electrochemical, fluorescent, and colorimetric properties. upon addition of external stimuli, including dna, thrombin, and atp, the specific interactions between targets and sensing elements could induce strand displacement reaction and conformation transformation, resulting in the integration of g-quadruplex/hemin complex as electrochemical probe, lighting up the fluorescence of dna/ag nanoclusters and enhancing the catalytic activity of dnazyme to catalyze the h2o2-tmb system to generate colorimetric signal. such a functional dna nanostructure actually serves as a biosensing platform, showing high sensitivity and selectivity for dna, thrombin, and atp detection. furthermore, we also show that this novel sensing platform can be utilized to detect three different kinds of targets independently and simultaneously. our integrated concept provides a paradigm for exploring the potential of twjs in analytical applications.
收录类别	SCI
语种	英语
WOS记录号	WOS:000365151000019
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/64844]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhang,Libing,Guo,Shaojun,Zhu,Jinbo,et al. Engineering DNA Three-Way Junction with Multifunctional Moieties: Sensing Platform for Bioanalysis[J]. analytical chemistry,2015,87(22):11295-11300.
APA	Zhang,Libing.,Guo,Shaojun.,Zhu,Jinbo.,Zhou,Zhixue.,Li,Tao.,...&Wang,Erkang.(2015).Engineering DNA Three-Way Junction with Multifunctional Moieties: Sensing Platform for Bioanalysis.analytical chemistry,87(22),11295-11300.
MLA	Zhang,Libing,et al."Engineering DNA Three-Way Junction with Multifunctional Moieties: Sensing Platform for Bioanalysis".analytical chemistry 87.22(2015):11295-11300.