A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors

	A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors
	Cao,Qiao; Teng,Ye; Yang,Xuan; Wang,Jin; Wang,Erkang
刊名	biosensors & bioelectronics
	2015
卷号	74 页码:318-321
关键词	SILVER NANOCLUSTERS LIVING CELLS METAL NANOCLUSTERS LOGIC DEVICES HYBRIDIZATION PROBE IMMOBILIZATION FLUOROPHORES CHALLENGES CLUSTERS
通讯作者	wang,e
英文摘要	in this paper, we developed a simple, low-cost and sensitive dna sequences detection biosensor based on a label-free molecular beacon (mb) whose dna hairpin structure terminal has a guanine-rich sequence that can enhance fluorescence of silver nanoclusters (ag ncs). without hybridization between hairpin probe and target dna, the ag ncs presented bright fluorescence for the proximity of guanine-rich sequences (grss). after binding with target dna, the hairpin shape was destroyed which results in a decrease of the ag ncs fluorescence intensity. with this biosensor, we detected three disease-related genes that were the human immunodeficiency virus (hiv) gene, hepatitis b virus (hbv) gene and human t-lymphotropic virus type i (htlv-i) gene. the detection limits based on s/n of 3 were 4.4 nm, 6.8 nm and 8.5 nm for hiv gene, hbv gene and htlv-i gene, respectively. our sensor was also of high selectivity and could distinguish even one nucleotide mismatched target. (c) 2015 elsevier b.v. all rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000360772800042
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/64770]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Cao,Qiao,Teng,Ye,Yang,Xuan,et al. A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors[J]. biosensors & bioelectronics,2015,74:318-321.
APA	Cao,Qiao,Teng,Ye,Yang,Xuan,Wang,Jin,&Wang,Erkang.(2015).A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors.biosensors & bioelectronics,74,318-321.
MLA	Cao,Qiao,et al."A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors".biosensors & bioelectronics 74(2015):318-321.