Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers

	Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers
	Bing, Tao 1,2; Shangguan, Dihua 1; Wang, Yinsheng 2
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2015-10-01
卷号	14 期号:10 页码:2692-2700
英文摘要	Cancer biomarker discovery constitutes a frontier in cancer research. In recent years, cell-binding aptamers have become useful molecular probes for biomarker discovery. However, there are few successful examples, and the critical barrier resides in the identification of the cell-surface protein targets for the aptamers, where only a limited number of aptamer targets have been identified so far. Herein, we developed a universal SILAC-based quantitative proteomic method for target discovery of cell-binding aptamers. The method allowed for distinguishing specific aptamer-binding proteins from nonspecific proteins based on abundance ratios of proteins bound to aptamer-carrying bait and control bait. In addition, we employed fluorescently labeled aptamers for monitoring and optimizing the binding conditions. We were able to identify and validate selectin L and integrin alpha 4 as the protein targets for two previously reported aptamers, Sgc-3b and Sgc-4e, respectively. This strategy should be generally applicable for the discovery of protein targets for other cell-binding aptamers, which will promote the applications of these aptamers.
收录类别	SCI
语种	英语
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28824]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA
推荐引用方式 GB/T 7714	Bing, Tao,Shangguan, Dihua,Wang, Yinsheng. Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers[J]. MOLECULAR & CELLULAR PROTEOMICS,2015,14(10):2692-2700.
APA	Bing, Tao,Shangguan, Dihua,&Wang, Yinsheng.(2015).Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers.MOLECULAR & CELLULAR PROTEOMICS,14(10),2692-2700.
MLA	Bing, Tao,et al."Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers".MOLECULAR & CELLULAR PROTEOMICS 14.10(2015):2692-2700.