Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 生物传感技术团队

	Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell
	Feng, Ruirui 1,2,3,4; Liang, Bo 3,4,5; Hou, Chuantao 3,4; Han, Dongfei 3,4; Han, Lei 3,4,5; Lang, Qiaolin 3,4; Liu, Aihua 3,4,5; Han, Lihui 1,2
刊名	ENZYME AND MICROBIAL TECHNOLOGY
	2016-03-01
卷号	84 页码:78-85
关键词	Homology modeling Xylose dehydrogenase mutants Thermostability Microbial surface display Xylose/O-2 based biofuel cell
英文摘要	In this paper, the construction of 3D model structure of xylose dehydrogenase (XDH) by using homology modeling to guide the rational design of the enzyme for improving thermostability was reported. Three XDH mutants of NA-1 (+249L), NA-2 (G149P) and NA-3 (+249L/G149P) were designed and displayed on the surface of bacteria. Among them, bacteria displaying NA-1 (NA-1-bacteria) exhibited superior thermostability without compromising its activity and substrate specificity in comparison with its wild-type counterpart. NA-1-bacteria retained its original activity after incubation at room temperature for one month with the half-life of 9.8 days at 40 degrees C. Finally, the NA-1-bacteria were applied to construct xylose/O-2 based biofuel cell with good performance including enhanced operational stability. Thus, the approach described here could be explored for engineering of other enzymes for improving certain characters without three-dimensional structure identified by experimental methods. (C) 2015 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	GLUCOSE-DEHYDROGENASE ; SURFACE DISPLAY ; WHOLE-CELL ; MODIFIED-ELECTRODE ; BIOSENSOR ; PROTEIN ; BACTERIA ; COIMMOBILIZATION ; METABOLISM ; SIMULATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000370306400010
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/8015]
专题	青岛生物能源与过程研究所_生物传感技术团队
作者单位	1.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, 238 Songling Rd, Qingdao 266100, Peoples R China 2.Ocean Univ China, Coll Chem & Chem Engn, 238 Songling Rd, Qingdao 266100, Peoples R China 3.Chinese Acad Sci, Lab Biosensing, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, 189 Songling Rd, Qingdao 266101, Peoples R China 5.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Feng, Ruirui,Liang, Bo,Hou, Chuantao,et al. Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell[J]. ENZYME AND MICROBIAL TECHNOLOGY,2016,84:78-85.
APA	Feng, Ruirui.,Liang, Bo.,Hou, Chuantao.,Han, Dongfei.,Han, Lei.,...&Han, Lihui.(2016).Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell.ENZYME AND MICROBIAL TECHNOLOGY,84,78-85.
MLA	Feng, Ruirui,et al."Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell".ENZYME AND MICROBIAL TECHNOLOGY 84(2016):78-85.