Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 代谢物组学团队

	Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin
	Liu, Ya-Jun 1,2,3; Song, Xiaxia 4; Li, Yifei 1,2,3; Xuan, Jinsong 4; Cui, Qiu 1,2,3; Wang, Jinfeng 5; Feng, Yingang 1,2,3
刊名	FEBS LETTERS
	2016-02-01
卷号	590 期号:3 页码:387-395
关键词	Mycobacterium tuberculosis NMR NrdH redoxin protein stability protein structure
英文摘要	NrdH redoxin is the only hydrogen donor for ribonucleotide reductase in Mycobacterium tuberculosis. Several crystal structures of NrdH redoxins in the oxidized state from different species have been reported, but no structure of the reduced state has yet been reported. Using NMR spectroscopy, we found surprisingly that the reduced NrdH redoxin from M. tuberculosis is largely unfolded at a pH lower than the pKa of its first active site cysteine, and the structural basis of the low stability was analyzed. In addition, a single mutant of the NrdH redoxin suitable to determine the structure in the reduced state was obtained.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]	N-15 NMR RELAXATION ; ESCHERICHIA-COLI ; ACTIVITY PROFILE ; PROTEIN ; C-13 ; DYNAMICS
收录类别	SCI
语种	英语
WOS记录号	WOS:000370136300009
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/8008]
专题	青岛生物能源与过程研究所_代谢物组学团队
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Synthet Biol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Beijing 100864, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Beijing 100864, Peoples R China 4.Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Dept Biol Sci & Engn, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ya-Jun,Song, Xiaxia,Li, Yifei,et al. Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin[J]. FEBS LETTERS,2016,590(3):387-395.
APA	Liu, Ya-Jun.,Song, Xiaxia.,Li, Yifei.,Xuan, Jinsong.,Cui, Qiu.,...&Feng, Yingang.(2016).Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin.FEBS LETTERS,590(3),387-395.
MLA	Liu, Ya-Jun,et al."Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin".FEBS LETTERS 590.3(2016):387-395.