Self-Assembled Virus-Like Particles from Rotavirus Structural Protein VP6 for Targeted Drug Delivery

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 张智军团队

	Self-Assembled Virus-Like Particles from Rotavirus Structural Protein VP6 for Targeted Drug Delivery
	Zhang, ZJ (张智军); Zhang, JP (张锦平); Zhang, ZJ (张智军)
刊名	BIOCONJUGATE CHEMISTRY
	2011-03
卷号	22 期号:3 页码:346-352
通讯作者	Zhang, ZJ (张智军)
英文摘要	Proteins of viral capsid may self-assemble into virus-like particles (VLPs) that can find many biomedical applications such as platform for drug delivery. In this paper, we describe preparation of VLPs by self-assembly of VP6, a rotavirus capsid protein that was chemically conjugated with doxorubicin (DOX), an anticancer drug. VP6 was first highly expressed in E. Coli, followed by purification and renaturation. DOX was then covalently attached to VP6 to form DOX-VP6 (DVP6) conjugates, which were subsequently self-assembled into VLPs under appropriate condition. Next, lactobionic acid (LA) was chemically linked to the surface of the VLPs. We demonstrated that the aforementioned nanosystem shows specific targeting to hepatoma cell line HepG2. The chemically functionalized VLPs, a kind of biological nanoparticles with excellent biocompatibility and biodegradability, can be prepared in large scale from E. Coli through our method, which may find practical applications in biomedicine.
收录类别	SCI
语种	英语
WOS记录号	WOS:000288401400005
公开日期	2012-08-22
内容类型	期刊论文
源URL	[http://58.210.77.100/handle/332007/557]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_张智军团队
通讯作者	Zhang, ZJ (张智军); Zhang, ZJ (张智军)
推荐引用方式 GB/T 7714	Zhang, ZJ ,Zhang, JP ,Zhang, ZJ . Self-Assembled Virus-Like Particles from Rotavirus Structural Protein VP6 for Targeted Drug Delivery[J]. BIOCONJUGATE CHEMISTRY,2011,22(3):346-352.
APA	Zhang, ZJ ,Zhang, JP ,&Zhang, ZJ .(2011).Self-Assembled Virus-Like Particles from Rotavirus Structural Protein VP6 for Targeted Drug Delivery.BIOCONJUGATE CHEMISTRY,22(3),346-352.
MLA	Zhang, ZJ ,et al."Self-Assembled Virus-Like Particles from Rotavirus Structural Protein VP6 for Targeted Drug Delivery".BIOCONJUGATE CHEMISTRY 22.3(2011):346-352.