Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 马宏伟团队

	Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors
	Ma, HW(马宏伟)
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2013-01-16
卷号	135 期号:2 页码:696-702
关键词	GOLD NANOPARTICLES FOLDING DNA NANOTUBES SHAPES
通讯作者	Liu, HJ
英文摘要	Self-assembled DNA origami nanostructures have shown great promise for bottom-up construction of complex objects with nanoscale addressability. Here we show that DNA origami-based ID nanoribbons and nanotubes are one-pot assembled with controllable sizes and nanoscale addressability with high speed (within only 10-20 min), exhibiting extraordinarily high cooperativity that is often observed in assembly of natural molecular machines in cells (e.g. ribosome). By exploiting the high specificity of DNA-based self-assembly, we can precisely anchor proteins on these DNA origami nanostructures with sub-10 nm resolution and at the single-molecule level. We attach a pair of enzymes (horseradish peroxidase and glucose oxidase) at the inner side of DNA nanotubes and observe high coupling efficiency of enzyme cascade within this confined nanospace. Hence, DNA nanostructures with such unprecedented properties shed new light on the design of nanoscale bioreactors and nanomedicine and provide an artificial system for studying enzyme activities and cascade in highly organized and crowded cell-mimicking environments.
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000313920800037
公开日期	2014-01-15
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/1397]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_马宏伟团队
推荐引用方式 GB/T 7714	Ma, HW. Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(2):696-702.
APA	Ma, HW.(2013).Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(2),696-702.
MLA	Ma, HW."Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.2(2013):696-702.