Time-resolved ICP-MS analysis of mineral element contents and distribution patterns in single cells

	Time-resolved ICP-MS analysis of mineral element contents and distribution patterns in single cells
	Wang HL(王海龙); Wang B(汪冰); Wang M(王萌); Zheng LN(郑令娜); Chen HQ(陈汉清); Chai ZF(柴之芳); Zhao YL(赵宇亮); Feng WY(丰伟悦); Wang, HL; Wang, B
刊名	ANALYST
	2015
卷号	140 期号:2 页码:523-531
通讯作者	丰伟悦
英文摘要	Novel single cell techniques are attracting growing interest for clinical applications, because they can elucidate the cellular diversity and heterogeneity instead of the average masked by bulk measurements. Herein, time-resolved ICP-MS for the determination of essential mineral elements in single cells has been developed and is used to analyze the contents and distribution patterns of Fe, Cu, Zn, Mn, P and S in two types of cancer cells (HeLa and A549) and one type of normal cells (16HBE). The results show that there are obvious differences in contents and distribution patterns of the elements among the three types of cells. The mass of Fe, Zn, Cu, Mn, P, and S in individual HeLa cells is significantly higher and span a broader range of values than in the single 16HBE and A549 cells. The contents of Fe, Zn, and Cu follow log-normal distributions, and Mn, P, and S follow Poisson distributions with high l values in single HeLa cells, indicating a large cell-to-cell variance. Comparatively, the contents of Cu, Zn, P, and S in 16HBE cells show the narrowest distribution range among the three tested cells, demonstrating the homogenous distribution of the elements in the cells. The method of single cell ICP-MS (SC-ICP-MS) provides potential applications for the monitoring of the variation of mineral elements at a single cell level.
学科主题	Chemistry
类目[WOS]	Chemistry, Analytical
收录类别	SCI ; CA ; MEDLINE
WOS记录号	WOS:000346304200017
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/228206]
专题	高能物理研究所_院士高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Wang HL,Wang B,Wang M,et al. Time-resolved ICP-MS analysis of mineral element contents and distribution patterns in single cells[J]. ANALYST,2015,140(2):523-531.
APA	王海龙.,汪冰.,王萌.,郑令娜.,陈汉清.,...&Feng, WY.(2015).Time-resolved ICP-MS analysis of mineral element contents and distribution patterns in single cells.ANALYST,140(2),523-531.
MLA	王海龙,et al."Time-resolved ICP-MS analysis of mineral element contents and distribution patterns in single cells".ANALYST 140.2(2015):523-531.