Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast

	Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast
	Li YF(李玉锋); Hu L(胡亮); Li B(李柏); Huang XH(黄小晗); Gao YX(高愈希); Chai ZF(柴之芳); Chen CY(陈春英); Li, YF; Hu, LA; Li, B
刊名	JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
	2011
卷号	26 期号:1 页码:#REF!
通讯作者	Chen, CY (reprint author), Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China.
英文摘要	Accurate determination of selenium (Se) species in biological samples is a critical issue because Se commonly occurs at low levels and in diverse species. The method for the full quantification of Se species in serum samples was proposed through combined ion-pair reverse-phase (RP) chromatography and affinity chromatography (AF) hyphenated to inductively coupled plasma( quadrupole) mass spectrometry (ICP-qMS) with post-column isotope dilution analysis (IDA) and a collision cell technique (CCT). Different Se species like inorganic Se (Se4+ and Se6+), selenocystine (SeCys), selenomethionine (SeMet), selenoprotein P (SelP), selenoalbumin (SeAlb) and glutathione peroxidase (GPx) can be separated and quantified. The proposed methodology was used to qualitatively and quantitatively study the dynamic distribution of Se species in human serum samples from the Hg-contaminated area after supplementation with 100 mu g of Se daily as Se-enriched yeast for 180 days. SelP takes up almost half and even more of the total Se and increases with the Se administration. The repeatability in terms of relative standard deviation (R. S. D. %, n = 10) is 6% for GPx and SelP and 5% for SeAlb. The detection limits are 0.1 mu g Se L-1 for GPx and other non-retained Se compounds, 1.0 mu g Se L-1 for SelP and 1.2 mu g Se L-1 for SeAlb, 1.3 mu g Se L-1 for inorganic Se; 1.2 mu g Se L-1 for SeCys; 1.1 mu g Se L-1 for SeMet, respectively.
学科主题	Chemistry; Spectroscopy
类目[WOS]	Chemistry, Analytical ; Spectroscopy
收录类别	SCI
WOS记录号	WOS:000285390600025
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/227047]
专题	高能物理研究所_院士高能物理研究所_实验物理中心高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Li YF,Hu L,Li B,et al. Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2011,26(1):#REF!.
APA	李玉锋.,胡亮.,李柏.,黄小晗.,高愈希.,...&Chen, CY.(2011).Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast.JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,26(1),#REF!.
MLA	李玉锋,et al."Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast".JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 26.1(2011):#REF!.