Roles for microtubule and microfilament cytoskeletons in animal cell cytokinesis

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	Roles for microtubule and microfilament cytoskeletons in animal cell cytokinesis
	Chen, ZC; Cai, S; Jiang, Q; Zhang, CM; Tang, XW
刊名	CHINESE SCIENCE BULLETIN
	2005
卷号	50 期号:3 页码:#REF!
关键词	cytokinesis microtubule actin Filament cytochalasin B nocodazole
通讯作者	Zhang, CM (reprint author), Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Natl Lab Biomembrane & Membrance Biotechnol, Beijing 100871, Peoples R China.
英文摘要	Microtubule and microfilament cytoskeletons play key roles in the whole process of cytokinesis. Although a number of hypotheses have been proposed to elucidate the mechanism of cytokinesis by microtubule and actin filament cytoskeletons, many reports are conflicting. In our study, combining the cytoskeletons drug treatments with the time-lapse video technology, we retested the key roles of microtubule and actin filament in cytokinesis. The results showed that depolymerization of microtubules by Nocodazole after the initiation of furrowing would not inhibit the furrow ingression, but obviously decrease the stiffness of daughter cells. Depolymerizing actin filaments by Cytochalasin B before metaphase would inhibit the initiation of furrowing but not chromosome segregation, resulting in the formation of binucleate cells; however, depolymerizing actin filaments during anaphase would prevent furrowing and lead to the regress of established furrow, also resulting in the formation of binucleate cells. Further, depolymerizing microtubules and actin filaments simultaneously after metaphase would cause the quick regress of the furrow and the formation of binucleate cells. From these results we propose that a successful cytokinesis requires functions and coordination of both the microtubule and actin filament cytoskeletons. Microtubule cytoskeleton may function in the positioning and initiation of cleavage furrow, and the actin filament cytoskeleton may play key roles in the initiation and ingression of the furrow.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
收录类别	SCI
WOS记录号	WOS:000228412100007
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/227076]
专题	高能物理研究所_粒子天体物理中心
推荐引用方式 GB/T 7714	Chen, ZC,Cai, S,Jiang, Q,et al. Roles for microtubule and microfilament cytoskeletons in animal cell cytokinesis[J]. CHINESE SCIENCE BULLETIN,2005,50(3):#REF!.
APA	Chen, ZC,Cai, S,Jiang, Q,Zhang, CM,&Tang, XW.(2005).Roles for microtubule and microfilament cytoskeletons in animal cell cytokinesis.CHINESE SCIENCE BULLETIN,50(3),#REF!.
MLA	Chen, ZC,et al."Roles for microtubule and microfilament cytoskeletons in animal cell cytokinesis".CHINESE SCIENCE BULLETIN 50.3(2005):#REF!.