Determination of low levels of polycyclic aromatic hydrocarbons in soil by high performance liquid chromatography with tandem fluorescence and diode-array detectors

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Determination of low levels of polycyclic aromatic hydrocarbons in soil by high performance liquid chromatography with tandem fluorescence and diode-array detectors
	Huang, Yujuan 1; Wei, Jing 1,2; Song, Jing 1,2; Chen, Mengfang 1,2; Luo, Yongming1,2,3
刊名	CHEMOSPHERE
	2013-08-01
卷号	92 期号:8 页码:1010-1016
关键词	HPLC FLD-DAD PAHs Soil
ISSN号	0045-6535
通讯作者	Song, J (reprint author) Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. jingsong@issas.ac.cn
英文摘要	Risk assessment of polycyclic aromatic hydrocarbons (PAHs) contaminated soil and source apportionment require accurate analysis of the concentration of each PAH congener in the soil. However, determination of low level PAH congeners in soil is difficult because of similarity in the chemical properties of 16 PAHs and severe matrix interferences due to complex composition of soils. It is therefore imperative to develop a sensitive and accurate method for determination of low level PAHs in soil. In this work, high performance liquid chromatography equipped with fluorescence and diode-array detectors (HPLC-FLD-DAD) was used to determine the concentration of 16 PAHs in soil. The separation of the 16 PAHs was achieved by optimization of the mobile phase gradient elution program and FLD wavelength switching program. Qualitative analysis of the 16 PM-Is was based on the retention time (RT) and each PAH specific spectrum obtained from DAD. In contrast, the quantitative analysis of individual PAH congeners was based on the peak areas at the specific wavelength with DAD and FLD. Under optimal conditions the detection limit was in the range 1.0-9.5 mu g L-1 for 16 PAHs with DAD and 0.01-0.1 mu g L-1 for 15 PAHs with FLD, and the RSD of PAHs was less than 5% with DAD and 3% with FLD. The spiked recoveries were in the range 61-96%, with the exception of NaP (<40%). The results show that HPLC-FLD-DAD can provide more accurate and reliable analysis of low level PAH congeners in soil samples. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Environmental Sciences & Ecology
研究领域[WOS]	Environmental Sciences & Ecology
关键词[WOS]	SOLID-PHASE EXTRACTION ; GAS-CHROMATOGRAPHY ; ULTRASONICATION EXTRACTION ; MASS SPECTROMETRY ; SURFACE SOILS ; HEALTH-RISK ; PRECONCENTRATION ; INJECTION ; MICROWAVE ; CLEANUP
收录类别	SCI
语种	英语
WOS记录号	WOS:000322428500019
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/9054]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室烟台海岸带研究所_近岸生态与环境实验室
作者单位	1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Yantai Inst Costal Zone Res, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yujuan,Wei, Jing,Song, Jing,et al. Determination of low levels of polycyclic aromatic hydrocarbons in soil by high performance liquid chromatography with tandem fluorescence and diode-array detectors[J]. CHEMOSPHERE,2013,92(8):1010-1016.
APA	Huang, Yujuan,Wei, Jing,Song, Jing,Chen, Mengfang,&Luo, Yongming.(2013).Determination of low levels of polycyclic aromatic hydrocarbons in soil by high performance liquid chromatography with tandem fluorescence and diode-array detectors.CHEMOSPHERE,92(8),1010-1016.
MLA	Huang, Yujuan,et al."Determination of low levels of polycyclic aromatic hydrocarbons in soil by high performance liquid chromatography with tandem fluorescence and diode-array detectors".CHEMOSPHERE 92.8(2013):1010-1016.