Rape (Brassica chinensis L.) seed germination, seedling growth, and physiology in soil polluted with di-n-butyl phthalate and bis(2-ethylhexyl) phthalate

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Rape (Brassica chinensis L.) seed germination, seedling growth, and physiology in soil polluted with di-n-butyl phthalate and bis(2-ethylhexyl) phthalate
	Ma, Tingting 1,2; Christie, Peter 3; Teng, Ying 1,2; Luo, Yongming1,2,4
刊名	ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
	2013-08-01
卷号	20 期号:8 页码:5289-5298
关键词	Phytotoxicity Oxidative stress PAEs Rape (Brassica rapa) Seed germination Seedling growth
ISSN号	0944-1344
通讯作者	Luo, YM (reprint author)Chinese Acad Sci, Yantai Inst Costal Zone Res, Yantai 264003, Peoples R China. ymluo@yic.ac.cn
英文摘要	Phthalic acid esters (PAEs) pollution in agricultural soils caused by widely employed plastic products is becoming more and more widespread in China. PAEs polluted soil can lead to phytotoxicity in higher plants and potential health risks to human being. We evaluated the individual toxicity of di-n-butyl phthalate (DnBP) and bis(2-ethylhexyl) phthalate (DEHP), two representative PAEs, to sown rape (Brassica chinensis L.) seeds within 72 h (as germination stage) and seedlings after germination for 14 days by monitoring responses and trends of different biological parameters. No significant effects of six concentrations of PAE ranging from 0 (not treated/NT) to 500 mg kg(-1) on germination rate in soil were observed. However, root length, shoot length, and biomass (fresh weight) were inhibited by both pollutants (except root length and biomass under DEHP). Stimulatory effects of both target pollutants on malondialdehyde (MDA) content, superoxide dismutase (SODase) activity, ascorbate peroxidase (APXase) content, and polyphenoloxidase (PPOase) activity in shoots and roots (SODase activity in shoots excluded) were in the same trend with the promotion of proline (Pro) but differed with acetylcholinesterase activity (except in shoots under DnBP) for analyzed samples treated for 72 h and 14 days. Responses of representative storage compounds free amino acids (FAA) and total soluble sugar (TSS) under both PAEs were raised. Sensitivity of APXase and Pro in roots demonstrates their possibility in estimation of PAE phytotoxicity and the higher toxicity of DnBP, which has also been approved by the morphological photos of seedlings at day 14. Higher sensitivity of the roots was also observed. The recommended soil allowable concentration is 5 mg DnBP kg(-1) soil for the development of rape. We still need to know the phytotoxicity of DEHP at whole seedling stage for both the growing and development; on the other hand, soil criteria for PAE compounds are urgently required in China.
学科主题	Environmental Sciences & Ecology
研究领域[WOS]	Environmental Sciences & Ecology
关键词[WOS]	LEAF POLYPHENOL OXIDASE ; ELSHOLTZIA-HAICHOWENSIS ; ANTIOXIDANT RESPONSES ; SUPEROXIDE-DISMUTASE ; LIPID-PEROXIDATION ; HYDROGEN-PEROXIDE ; OXIDATIVE STRESS ; PLANTS ; ESTERS ; ACETYLCHOLINESTERASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000321908600020
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/9029]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Soil Environm & Pollut Remediat, Inst Soil Sci, Nanjing 210008, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland 4.Chinese Acad Sci, Yantai Inst Costal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Ma, Tingting,Christie, Peter,Teng, Ying,et al. Rape (Brassica chinensis L.) seed germination, seedling growth, and physiology in soil polluted with di-n-butyl phthalate and bis(2-ethylhexyl) phthalate[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2013,20(8):5289-5298.
APA	Ma, Tingting,Christie, Peter,Teng, Ying,&Luo, Yongming.(2013).Rape (Brassica chinensis L.) seed germination, seedling growth, and physiology in soil polluted with di-n-butyl phthalate and bis(2-ethylhexyl) phthalate.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,20(8),5289-5298.
MLA	Ma, Tingting,et al."Rape (Brassica chinensis L.) seed germination, seedling growth, and physiology in soil polluted with di-n-butyl phthalate and bis(2-ethylhexyl) phthalate".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 20.8(2013):5289-5298.