ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells
	Wang, CX; Hu, XK; Gao, Y; Ji, YL
刊名	BIOMED RESEARCH INTERNATIONAL
	2015
ISSN号	2314-6133
通讯作者	Hu, XK (reprint author), Chinese Acad Sci, Yantal Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, 17 Chunhui Rd, Yantai 264003, Peoples R China. xkhu@yic.ac.cn
产权排序	[Wang, Caixia; Hu, Xiaoke] Chinese Acad Sci, Yantal Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China; [Wang, Caixia] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Gao, Yan] Natl Oceanog Ctr, Qingdao 266071, Peoples R China; [Ji, Yinglu] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
中文摘要	Owing to the wide use of novel nanoparticles (NPs) such as zinc oxide (ZnO) in all aspects of life, toxicological research on ZnO NPs is receiving increasing attention in these days. In this study, the toxicity of ZnO NPs in a human pulmonary adcnocarcinoma cell line 1_,TEP-a-2 was tested in vitro. Log-phase cells were exposed to different levels of ZnO NPs for hours, followed by colorimetric cell viability assay using tetrazoliwn salt and cell survival rate assay using trypan blue dye. Cell morphological changes were observed by Giemsa staining and light microscopy. Apoptosis was detected by using fluorescence microscopy and caspase-3 activity assay. Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method. Results showed that ZnO NPs (0.011.1g/mL) significantly inhibited proliferation (P < 0.05) and induced substantial apoptosis in 1,TEP-a-2 cells after 4 h of exposure. The intracellular ROS level rose up to 30-40% corresponding to significant depletion (approximately 70-80%) in GSH content in LTEP-a-2 cells (P < 0.05), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production. This study elucidates the toxicological mechanism of ZnO NPs in human pulmonary adenocarcinoma cells and provides reference data for application of nanomaterials in the environment.
英文摘要	Owing to the wide use of novel nanoparticles (NPs) such as zinc oxide (ZnO) in all aspects of life, toxicological research on ZnO NPs is receiving increasing attention in these days. In this study, the toxicity of ZnO NPs in a human pulmonary adcnocarcinoma cell line 1_,TEP-a-2 was tested in vitro. Log-phase cells were exposed to different levels of ZnO NPs for hours, followed by colorimetric cell viability assay using tetrazoliwn salt and cell survival rate assay using trypan blue dye. Cell morphological changes were observed by Giemsa staining and light microscopy. Apoptosis was detected by using fluorescence microscopy and caspase-3 activity assay. Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method. Results showed that ZnO NPs (0.011.1g/mL) significantly inhibited proliferation (P < 0.05) and induced substantial apoptosis in 1,TEP-a-2 cells after 4 h of exposure. The intracellular ROS level rose up to 30-40% corresponding to significant depletion (approximately 70-80%) in GSH content in LTEP-a-2 cells (P < 0.05), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production. This study elucidates the toxicological mechanism of ZnO NPs in human pulmonary adenocarcinoma cells and provides reference data for application of nanomaterials in the environment.
研究领域[WOS]	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
关键词[WOS]	OXIDATIVE STRESS ; PULMONARY TOXICITY ; IN-VITRO ; PARTICLES ; PATHWAYS ; BACTERIA
收录类别	SCI
语种	英语
WOS记录号	WOS:000359551100001
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/9284]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
推荐引用方式 GB/T 7714	Wang, CX,Hu, XK,Gao, Y,et al. ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells[J]. BIOMED RESEARCH INTERNATIONAL,2015.
APA	Wang, CX,Hu, XK,Gao, Y,&Ji, YL.(2015).ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells.BIOMED RESEARCH INTERNATIONAL.
MLA	Wang, CX,et al."ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells".BIOMED RESEARCH INTERNATIONAL (2015).