Se-Hg Dual-element Labeling Strategy for Selectively Recognizing Selenoprotein and Selenopeptide

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Se-Hg Dual-element Labeling Strategy for Selectively Recognizing Selenoprotein and Selenopeptide
	Xu Ming; Yang Li-Min; Wang Qiu-Quan
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2015-08
卷号	43 期号:9 页码:1265-1271
关键词	Selenium Mercury Selenoprotein selenopeptide Inductively coupled plasma mass spectrometry
英文摘要	An endogenous element-label plus exogenous element-tag strategy was proposed for inductively coupled plasma-mass spectrometry (ICP-MS) to screen and discriminate a family of ultratrace but biologically important biomolecules. The feasibility of this novel idea was demonstrated by setting seleno (SeCys) and Se-containing (SeMet) proteins (peptides) as an example. Se-label naturally occurring in the biomolecules acted as an identifier for picking up them out of a large amount of various coexisting proteins (peptides), and CH3Hg-tag that could bind to SeCys rather than SeMet helped discriminating seleno and Se-containing ones simply based on the Se and Hg signals on ICP-MS. This strategy has been applied to screen and discriminate Seleno and Se-containing proteins (peptides) in water-soluble extracts of Se-enriched yeast, and seven selenoproteins (peptides) were detected with both Hg-202 and Se-82 signals out of fifteen Se-containing species using RPLC/ICP-MS, providing valuable information for further identification using a high-resolution structure-selective mass spectrometer. This endogenous element-label plus exogenous element-tag dual-element approach implies that ICP-MS is not only able to quantify targeted proteins (peptides) but also helpful to discover unknown ones during a discovery-based proteomic study.
研究领域[WOS]	Chemistry, Analytical
WOS记录号	WOS:000361628600001
公开日期	2016-03-15
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/32657]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Xu Ming,Yang Li-Min,Wang Qiu-Quan. Se-Hg Dual-element Labeling Strategy for Selectively Recognizing Selenoprotein and Selenopeptide[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2015,43(9):1265-1271.
APA	Xu Ming,Yang Li-Min,&Wang Qiu-Quan.(2015).Se-Hg Dual-element Labeling Strategy for Selectively Recognizing Selenoprotein and Selenopeptide.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,43(9),1265-1271.
MLA	Xu Ming,et al."Se-Hg Dual-element Labeling Strategy for Selectively Recognizing Selenoprotein and Selenopeptide".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 43.9(2015):1265-1271.