N-6-Methyladenine DNA Modification in Drosophila

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	N-6-Methyladenine DNA Modification in Drosophila
	Zhang, Guoqiang; Huang, Hua; Liu, Di; Cheng, Ying; Liu, Xiaoling; Zhang, Wenxin; Yin, Ruichuan; Zhang, Dapeng; Zhang, Peng; Liu, Jianzhao
刊名	CELL
	2015-05-07
卷号	161 期号:4 页码:893-906
英文摘要	DNA N-6-methyladenine (6mA) modification is commonly found in microbial genomes and plays important functions in regulating numerous biological processes in bacteria. However, whether 6mA occurs and what its potential roles are in higher-eukaryote cells remain unknown. Here, we show that 6mA is present in Drosophila genome and that the 6mA modification is dynamic and is regulated by the Drosophila Tet homolog, DNA 6mA demethylase ( DMAD), during embryogenesis. Importantly, our biochemical assays demonstrate that DMAD directly catalyzes 6mA demethylation in vitro. Further genetic and sequencing analyses reveal that DMAD is essential for development and that DMAD removes 6mA primarily from transposon regions, which correlates with transposon suppression in Drosophila ovary. Collectively, we uncover a DNA modification in Drosophila and describe a potential role of the DMAD-6mA regulatory axis in controlling development in higher eukaryotes.
研究领域[WOS]	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000354175200021
公开日期	2016-03-15
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/32626]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zhang, Guoqiang,Huang, Hua,Liu, Di,et al. N-6-Methyladenine DNA Modification in Drosophila[J]. CELL,2015,161(4):893-906.
APA	Zhang, Guoqiang.,Huang, Hua.,Liu, Di.,Cheng, Ying.,Liu, Xiaoling.,...&Chen, Dahua.(2015).N-6-Methyladenine DNA Modification in Drosophila.CELL,161(4),893-906.
MLA	Zhang, Guoqiang,et al."N-6-Methyladenine DNA Modification in Drosophila".CELL 161.4(2015):893-906.