Low concentrations of 17 beta-trenbolone induce female-to-male reversal and mortality in the frog Pelophylax nigromaculatus

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Low concentrations of 17 beta-trenbolone induce female-to-male reversal and mortality in the frog Pelophylax nigromaculatus
	Li, Yuan-Yuan; Xu, Wei; Chen, Xiao-Ran; Lou, Qin-Qin; Wei, Wu-Ji; Qin, Zhan-Fen
刊名	AQUATIC TOXICOLOGY
	2015-01
卷号	158 期号:1 页码:230-237
关键词	17 beta-trenbolone Pelophylax nigromaculatus Female-to-male reversal Environmental androgen Ovary-biased gene
英文摘要	Trenbolone, as a growth promoter in animal agriculture, has become an environmental androgen in surface water. Here, we aimed to reveal the effects of 17 beta-trenbolone on survival, growth, and gonadal differentiation in the frog Pelophylax nigromaculatus, which is widespread in East Asia and undergoing population decline. P. nigromaculatus tadpoles were exposed to 17 beta-trenbolone (0.1, 1, 10 mu g/L) from Gosner stage 24/25 to complete metamorphosis. We found that 17 beta-trenbolone resulted in significantly high mortality in a concentration-dependent manner, with a decrease in body weight in the high concentration group compared with the solvent control. Based on gross gonadal morphology, no females were observed, instead of about 15% ambiguous sexes and 85% males, in all 17 beta-trenbolone treatment groups. Like normal testes, the gonads with sex-ambiguous morphology exhibited testicular histology, showing that the sex-ambiguous gonads were incomplete ovary-to-testis reversals (IOTTRs) with certain ovarian morphological features. In the IOTTRs, the transcriptional levels of ovary-biased genes decreased drastically relative to normal ovaries, and even declined to the levels in normal testes. These observations confirmed that all test concentrations of 17 beta-trenbolone resulted in 100% sex reversal, although some sex-reversed testes retained some ovarian characteristics at the morphological level. To our knowledge, this is the first repcirt strongly demonstrating that trenbolone can cause female-to-male reversal in amphibians. Given that the lowest concentration tested is environmentally relevant, our study highlights the risks of trenbolone and other environmental androgens for P. nigromaculatus and other amphibians, in particular the species with high sensitivity of gonadal differentiation to androgenic chemicals. (C) 2014 Elsevier B.V. All rights reserved.
研究领域[WOS]	Marine & Freshwater Biology ; Toxicology
WOS记录号	WOS:000348888400025
公开日期	2016-03-15
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/32614]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Li, Yuan-Yuan,Xu, Wei,Chen, Xiao-Ran,et al. Low concentrations of 17 beta-trenbolone induce female-to-male reversal and mortality in the frog Pelophylax nigromaculatus[J]. AQUATIC TOXICOLOGY,2015,158(1):230-237.
APA	Li, Yuan-Yuan,Xu, Wei,Chen, Xiao-Ran,Lou, Qin-Qin,Wei, Wu-Ji,&Qin, Zhan-Fen.(2015).Low concentrations of 17 beta-trenbolone induce female-to-male reversal and mortality in the frog Pelophylax nigromaculatus.AQUATIC TOXICOLOGY,158(1),230-237.
MLA	Li, Yuan-Yuan,et al."Low concentrations of 17 beta-trenbolone induce female-to-male reversal and mortality in the frog Pelophylax nigromaculatus".AQUATIC TOXICOLOGY 158.1(2015):230-237.