Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens

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	Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens
	Lou, XD; Zhuang, Y; Zuo, XL; Jia, YM; Hong, YN; Min, XH; Zhang, ZY; Xu, XM; Liu, NN; Xia, F
刊名	ANALYTICAL CHEMISTRY
	2015
卷号	87 期号:13 页码:6822—6827
关键词	AGGREGATION-INDUCED EMISSION REPEAT AMPLIFICATION PROTOCOL HIGHLY SENSITIVE DETECTION IN-SITU INTRACELLULAR TELOMERASE QUANTUM DOTS LIVING CELLS ASSAY-METHOD TUMOR-CELLS DNA
ISSN号	0003-2700
通讯作者	Xia, F (reprint author), Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Key Lab Large Format Battery Mat & Syst, Minist Educ, Wuhan 430074, Peoples R China.
英文摘要	As a biomarker for early cancer diagnosis, telomerase are one of the promising targets for cancer therapeutics. Inspired by the fluorescent emission principle of aggregation-induced emission fluorogens, we creatively designed an AIE-based turn-on method to detect telomerase activity from cell extracts. A positively charged fluorogen (TPE-Z) is not fluorescent when freely diffused in solution. The fluorescence of TPE-Z is enhanced with the elongation of the DNA strand which could light up telomere elongation process. By exploitation of it, we can detect telomerase activity from different cell lines (E-J, HeLa, MCF-7, and HLF) with high sensitivity and specificity. Moreover, our method is successfully employed to demonstrate the applications in bladder cancer diagnosis (41 urine specimens from bladder cancer patients and 15 urine specimens from normal people are detected). The ATE-based method provides a simple one-pot technique for quantification and monitoring of the telomerase activity and shows great potential for future use in clinical tests.
语种	英语
WOS记录号	WOS:000357839700059
公开日期	2016-03-08
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/25029]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Lou, XD,Zhuang, Y,Zuo, XL,et al. Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens[J]. ANALYTICAL CHEMISTRY,2015,87(13):6822—6827.
APA	Lou, XD.,Zhuang, Y.,Zuo, XL.,Jia, YM.,Hong, YN.,...&Tang, BZ.(2015).Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens.ANALYTICAL CHEMISTRY,87(13),6822—6827.
MLA	Lou, XD,et al."Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens".ANALYTICAL CHEMISTRY 87.13(2015):6822—6827.