Differential expression of extracellular thiol groups of moderately thermophilic Sulfobacillus thermosulfidooxidans and extremely thermophilic Acidianus manzaensis grown on S-0 and Fe2+

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	Differential expression of extracellular thiol groups of moderately thermophilic Sulfobacillus thermosulfidooxidans and extremely thermophilic Acidianus manzaensis grown on S-0 and Fe2+
	Liu, HC; Xia, JL; Nie, ZY; Zhen, XJ; Zhang, LJ
刊名	ARCHIVES OF MICROBIOLOGY
	2015
卷号	197 期号:6 页码:823—831
关键词	BACTERIUM ALLOCHROMATIUM-VINOSUM ACIDITHIOBACILLUS-FERROOXIDANS SULFUR-COMPOUNDS ELEMENTAL SULFUR OXIDATION PROTEINS TRANSFORMATION CHALCOPYRITE MICROSCOPE METABOLISM
ISSN号	0302-8933
通讯作者	Xia, JL (reprint author), Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.
英文摘要	Bio-oxidation of elemental sulfur (S-0) is very important in bioleaching and sulfur cycle. S-0 was proposed to be first activated by reacting with reactive thiol groups (-SH) of outer membrane proteins, forming -S (n) H (n a parts per thousand yen 2) complexes. The differential expression of -SH of moderately thermophilic Sulfobacillus thermosulfidooxidans and extremely thermophilic Acidianus manzaensis grown on Fe2+ and S-0 was investigated by synchrotron radiation-based scanning transmission X-ray microscopy (STXM) imaging and micro-beam X-ray fluorescence (mu-XRF) mapping. The STXM imaging and mu-XRF mapping of extracellular -SH were based on the analysis of Ca2+ bound on the cell. By comparing Ca2+ of the cells with and without labeling by Ca2+, the distribution and content of thiol groups were obtained. The results showed that, for both S. thermosulfidooxidans and A. manzaensis, the expression of extracellular -SH of S-0-grown cells was higher than that of Fe2+-grown cells. Statistical analysis indicated that the expression of extracellular -SH for S. thermosulfidooxidans and A. manzaensis grown on S-0 was 2.37 times and 2.14 times, respectively, to that on Fe2+. These results evidently demonstrate that the extracellular thiol groups are most probably involved in elemental sulfur activation and oxidation of the acidophilic sulfur-oxidizing microorganisms.
收录类别	SCI
语种	英语
WOS记录号	WOS:000358086200011
公开日期	2016-03-08
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/24899]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Liu, HC,Xia, JL,Nie, ZY,et al. Differential expression of extracellular thiol groups of moderately thermophilic Sulfobacillus thermosulfidooxidans and extremely thermophilic Acidianus manzaensis grown on S-0 and Fe2+[J]. ARCHIVES OF MICROBIOLOGY,2015,197(6):823—831.
APA	Liu, HC,Xia, JL,Nie, ZY,Zhen, XJ,&Zhang, LJ.(2015).Differential expression of extracellular thiol groups of moderately thermophilic Sulfobacillus thermosulfidooxidans and extremely thermophilic Acidianus manzaensis grown on S-0 and Fe2+.ARCHIVES OF MICROBIOLOGY,197(6),823—831.
MLA	Liu, HC,et al."Differential expression of extracellular thiol groups of moderately thermophilic Sulfobacillus thermosulfidooxidans and extremely thermophilic Acidianus manzaensis grown on S-0 and Fe2+".ARCHIVES OF MICROBIOLOGY 197.6(2015):823—831.